Kamalati T, McIvor Z, Howard M, Green M R, Brooks R F
Department of Anatomy and Human Biology, Kings College London, United Kingdom.
Exp Cell Res. 1989 Dec;185(2):453-63. doi: 10.1016/0014-4827(89)90314-5.
SVK14, an SV40-transformed human keratinocyte line, has previously been reported to be almost completely unable to differentiate, and indeed, to express a set of keratins characteristic of simple epithelia rather than the stratifying epithelium from which they were derived. We have recently shown that IGF I stimulation of SVK14 results in expression of keratin 14, a marker of stratifying epithelia, as well as expression of markers which are characteristic of differentiation in normal human keratinocytes such as involucrin and keratin 10. To study further the capacity of SVK14 to differentiate, we have cocultured SVK14 with a variety of fibroblastic cell lines with a view to examining whether the cocultured partner can promote or interfere with their differentiation. We have observed that SVK14, when cocultured with Swiss 3T3, form organized structures through specific cell-cell interactions in which SVK14 express keratins 14 and 5 and involucrin, while maintaining T-antigen expression. These results are interesting since they show coculturing of a transformed human keratinocyte cell line and a particular fibroblast line can result in induction of characteristics of stratifying epithelia in a cell line with characteristics of simple epithelia. This may be analogous to the epithelial-mesenchymal interactions seen during epithelial development in the very early embryo.
SVK14是一种经SV40转化的人角质形成细胞系，此前有报道称其几乎完全无法分化，实际上，它表达的是一组简单上皮细胞特有的角蛋白，而非其来源的分层上皮细胞的角蛋白。我们最近发现，用胰岛素样生长因子I（IGF I）刺激SVK14会导致分层上皮细胞标志物角蛋白14的表达，以及正常人角质形成细胞分化特征性标志物如兜甲蛋白和角蛋白10的表达。为了进一步研究SVK14的分化能力，我们将SVK14与多种成纤维细胞系共培养，以考察共培养的细胞伙伴是否能促进或干扰其分化。我们观察到，当SVK14与瑞士3T3细胞共培养时，通过特定的细胞间相互作用形成有组织的结构，其中SVK14表达角蛋白14和5以及兜甲蛋白，同时维持T抗原的表达。这些结果很有意思，因为它们表明，将一种转化的人角质形成细胞系与一种特定的成纤维细胞系共培养，可导致具有简单上皮细胞特征的细胞系诱导出分层上皮细胞的特征。这可能类似于在胚胎极早期上皮发育过程中所见的上皮 - 间充质相互作用。
