Taylor-Papadimitriou J, Purkis P, Lane E B, McKay I A, Chang S E
Cell Differ. 1982 May;11(3):169-80. doi: 10.1016/0045-6039(82)90008-2.
A cloned cell line (SVK14) with apparently unlimited growth potential was isolated from simian virus 40 (SV40)-infected human foreskin keratinocytes which did not appear to pass through any obvious 'crisis' (Girardi et al., J. Cell. Comp. Physiol., 1965, 65, 69-84). Indirect immunofluorescence microscopy showed that the transformed cells have the SV40 large T antigen in their nuclei and stain positively with LE61, a monoclonal antibody that reacts with a tonofilament determinant normally only found in non-keratinizing simple epithelia. SVK14 cells can be grown in the absence of 3T3 feeders and show an impaired ability to differentiate into squames, and this impairment becomes more marked with passage. At later passages the cells acquire the ability to form colonies in agar and to produce a factor with mitogenic activity which stimulates DNA synthesis in quiescent 3T3 cells. Concomitantly, the SVK14 cells become less sensitive to the growth inhibitory effect of human alpha interferons.
从感染猿猴病毒40（SV40）的人包皮角质形成细胞中分离出一种具有明显无限生长潜能的克隆细胞系（SVK14），这些细胞似乎未经历任何明显的“危机”（吉拉尔迪等人，《细胞与比较生理学杂志》，1965年，65卷，69 - 84页）。间接免疫荧光显微镜检查显示，转化细胞的细胞核中有SV40大T抗原，并且用LE61呈阳性染色，LE61是一种单克隆抗体，它与一种通常仅在非角化复层扁平上皮中发现的张力丝决定簇发生反应。SVK14细胞可以在没有3T3饲养细胞的情况下生长，并且显示出分化为鳞屑的能力受损，随着传代这种损伤变得更加明显。在后期传代时，细胞获得了在琼脂中形成集落的能力以及产生具有促有丝分裂活性的因子的能力，该因子可刺激静止的3T3细胞中的DNA合成。同时，SVK14细胞对人α干扰素的生长抑制作用变得不那么敏感。
