2012年全国寄生虫病诊断技术竞赛分析报告：一、疟原虫检测能力分析

[Analysis report of the national technique competition for diagnosis of parasitic diseases in 2012: I. Capability analysis of Plasmodium detection].

作者信息

Zhang Shao-Sen, Xia Zhi-Gui, Yin Jian-Hai, Yan He, Zhou Shui-Sen, Li Shi-Zhu, Zheng Xiang, Huang Fang, Li Mei, Chen Hai-Tang, Wang Qiang, Zhang Li, Liu Wei, Xiao Ning, Zhou Xiao-Nong

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2013 Apr;31(2):131-4.

PMID:24809195

Abstract

OBJECTIVE

To analyze the result of the national technique competition for diagnosis of parasitic diseases in 2012, so as to understand the capability of detection on Plasmodium parasites among professionals from institutes for disease control and prevention at different levels.

METHODS

Four professionals from institution were selected as contestants (age < or = 45 and at least two contestants from county-level institution). The content of the competition included making thick and thin blood slides of Plasmodium (3 slides in 30 min, 10 scores as full marks and 6 as passing score) and identification of species and number with microscopy (5 slides, 8 min per slide, 30 scores as full marks and 18 as passing score). All contestants were grouped by gender, age, professional title, level of institution, classification according to malaria endemicity, geographical location and economic development of the province. Their scores were statistically analyzed by SPSS 16.0 software.

RESULTS

The average score of blood smear making test in 120 contestants from 30 provinces was 8.7, the highest was 10 and the lowest was 5.8, 118 (98.3%) contestants passed the test. The average score of blood smear reading was 16.0, the highest was 29 and the lowest was 0, 52 (43.3%) contestants passed the test. There were no significant differences for the scores among genders, ages (< or = 30, 31-40, > 40), professional titles (junior, intermediate and senior), institution levels (provincial, municipal or county level) (P > 0.05). However, there was a significant difference among provinces with different malaria endemicity, geographical location and development status (P < 0.05). For the blood slide-making and film-reading, scores of contestants from malaria endemic provinces including Class I (9.29 +/- 0.41, 18.17 +/- 6.42), Class II (8.92 +/- 0.79, 18.31 +/- 6.94) and Class III (8.61 +/- 0.89, 15.63 +/- 7.52) were higher than those from non-endemic provinces (7.95 +/- 1.00, 10.19 +/- 7.01) (P < 0.01). Scores of contestants from southern provinces (9.16 +/- 0.61, 18.82 +/- 6.78) were significantly higher than that from northern ones (8.30 +/- 0.99, 13.23 +/- 7.45) (P < 0.01). The film-reading scores were significantly higher in those from eastern provinces (18.20 +/- 6.88) than those from western (13.39 +/- 7.60) (P < 0.05), while no significant difference was found in blood slide-making (P > 0.05).

CONCLUSION

The capability of malaria parasite detection is imbalanced.

摘要

目的

分析2012年全国寄生虫病诊断技术竞赛结果，了解不同级别疾病预防控制机构专业人员对疟原虫的检测能力。

方法

从各机构选拔4名专业人员作为参赛选手（年龄≤45岁，且至少有2名来自县级机构）。竞赛内容包括制作疟原虫厚、薄血涂片（30分钟内制作3张涂片，满分10分，及格分数为6分）以及显微镜下疟原虫种类和数量的识别（5张涂片，每张涂片8分钟，满分30分，及格分数为18分）。所有参赛选手按性别、年龄、职称、机构级别、疟疾流行程度分类、地理位置和所在省份的经济发展情况进行分组。采用SPSS 16.0软件对其成绩进行统计学分析。

结果

来自30个省份的120名参赛选手血涂片制作测试的平均成绩为8.7分，最高分为10分，最低分为5.8分，118名（98.3%）选手通过测试。血涂片镜检平均成绩为16.0分，最高分为29分，最低分为0分，52名（43.3%）选手通过测试。性别、年龄（≤30岁、31 - 40岁、＞40岁）、职称（初级、中级和高级）、机构级别（省级、市级或县级）之间的成绩差异无统计学意义（P＞0.05）。然而，不同疟疾流行程度、地理位置和发展状况的省份之间存在显著差异（P＜0.05）。对于血涂片制作和镜检成绩，来自疟疾流行省份I类（9.29±0.41，18.17±6.42）、II类（8.92±0.79，18.31±6.94）和III类（8.61±0.89，15.63±7.52）的参赛选手成绩高于非流行省份（7.95±1.00，10.19±7.01）（P＜0.01）。来自南方省份的参赛选手成绩（9.16±0.61，18.82±6.78）显著高于北方省份（8.30±0.99，13.23±7.45）（P＜0.01）。东部省份参赛选手的镜检成绩（18.20±6.88）显著高于西部省份（13.39±7.60）（P＜0.05），而血涂片制作成绩差异无统计学意义（P＞0.05）。