Chiu William Chien-Hsien, Yeh Wen-Bin, Chen Mei-Er, Yang Man-Miao
a Laboratory of Molecular Systematics, Department of Entomology .
b Laboratory of Insect Molecular Biology, Department of Entomology , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):686-7. doi: 10.3109/19401736.2014.913143. Epub 2014 May 9.
Aeolesthes oenochrous (Fairmaire), a large and colorful longhorn beetle, is an endangered species in Taiwan. Its complete mitogenome, 15,747 bp, shows a typical coleopteran organization, containing 13 protein coding genes, 22 tRNA genes, 2 rRNA genes and one A + T rich region. Two protein coding genes, i.e. COI and ND1, have the atypical start codon of AAT and TTG, respectively. The third nucleotide position of codons shows extremely low guanine content. In the A + T rich region, there were two poly-T stretches with 14 and 13 thymine each. These two poly-T stretches were clarified by the cloning method.
台湾大紫斑蝶(Aeolesthes oenochrous (Fairmaire))是一种大型且色彩斑斓的天牛,是台湾的濒危物种。其完整的线粒体基因组长度为15747 bp,呈现出典型的鞘翅目结构,包含13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一个富含A + T的区域。两个蛋白质编码基因,即COI和ND1,分别具有非典型的起始密码子AAT和TTG。密码子的第三个核苷酸位置显示鸟嘌呤含量极低。在富含A + T的区域,有两个各含14个和13个胸腺嘧啶的多聚T序列。通过克隆方法明确了这两个多聚T序列。