Zambon Joao Paulo, de Sá Barretto Letícia Siqueira, Nakamura Ahy Nathally Sawaki E, Duailibi Silvio, Leite Kátia, Magalhaes Renata S, Orlando Giuseppe, Ross Christina L, Peloso Andrea, Almeida Fernando G
Federal University of Sao Paulo; Sao Paulo, Brazil; Wake Forest Institute for Regenerative Medicine; Winston-Salem, NC USA.
Federal University of Sao Paulo; Sao Paulo, Brazil.
Organogenesis. 2014 Apr-Jun;10(2):278-88. doi: 10.4161/org.29058. Epub 2014 May 22.
To evaluate the morphological and histological changes induced by PGA scaffold seeded with autologous adipose or muscle derived stem cells implanted on rabbit bladder wall.
Adipose derived stem cells (ADSCs) were obtained from the inguinal fat of eight rabbits and muscle derived stem cells (MDSCs) from the anterior tibial muscle of other eight rabbits. After culture and isolation, the cells were stained with Vybrant Red CM DiI and then implanted at third passage. Two PGA scaffolds were implanted on the bladder submucosa of each animal. On the right bladder side was implanted unseeded PGA scaffold while on the left side was implanted ADSCs or skeletal MDSCs seeded PGA scaffold. ADSCs were implanted in eight animals and MDSC in other eight animals. The animals were sacrificed at four and eight weeks. Histological evaluation was performed with Hematoxylin and Eosin, Masson's Trichrome and smooth muscle α-actin.
We observed a mild inflammatory response in all the three groups. Seeded scaffolds induced higher lymphocytes and lower polimorphonuclear migration than controls. Fibrosis was more pronounced in the control groups. Smooth muscle α-actin was positive only in ADSC and MDSC seeded scaffolds. At four and eight weeks ADCSs and skeletal MDSCs labeled cells were found at the implant sites.
The implantation of PGA scaffolds seeded with ADSC and MDSC induced less fibrosis than control and smooth muscle regeneration.
评估接种自体脂肪或肌肉来源干细胞的聚乙醇酸（PGA）支架植入兔膀胱壁后引起的形态学和组织学变化。
从8只兔的腹股沟脂肪中获取脂肪来源干细胞（ADSCs），从另外8只兔的胫骨前肌中获取肌肉来源干细胞（MDSCs）。培养和分离后，细胞用Vybrant Red CM DiI染色，然后在第三代时植入。将两个PGA支架植入每只动物的膀胱黏膜下层。在膀胱右侧植入未接种细胞的PGA支架，在左侧植入接种ADSCs或骨骼肌MDSCs的PGA支架。8只动物植入ADSCs，另外8只动物植入MDSCs。在4周和8周时处死动物。用苏木精和伊红、Masson三色染色法和平滑肌α-肌动蛋白进行组织学评估。
我们在所有三组中均观察到轻度炎症反应。接种细胞的支架比对照组诱导出更高的淋巴细胞浸润和更低的多形核细胞迁移。纤维化在对照组中更明显。平滑肌α-肌动蛋白仅在接种ADSC和MDSC的支架中呈阳性。在4周和8周时，在植入部位发现了标记的ADSCs和骨骼肌MDSCs细胞。
接种ADSC和MDSC的PGA支架植入比对照组诱导的纤维化更少且有平滑肌再生。
