多层支架上种植的干细胞植入受损的膀胱大鼠模型可改善膀胱功能。
Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function.
机构信息
1Department of Urology, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591 Republic of Korea.
2Cancer Research Institute, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591 Republic of Korea.
出版信息
Tissue Eng Regen Med. 2019 Mar 22;16(2):201-212. doi: 10.1007/s13770-019-00187-x. eCollection 2019 Apr.
BACKGROUND
To investigate whether human adipose-derived stem cells (hADSCs) seeded on multilayered poly (l-lactide--ɛ-caprolactone) (PLCL) sheets improve bladder function in a rat model of detrusor smooth muscle-removed bladder.
METHODS
Male rats were randomly divided into 4 groups: Normal, injury (detrusor smooth muscle-removed bladder), PLCL (detrusor smooth muscle-removed bladder implanted with PLCL sheets), and PLCL + ADSC (detrusor smooth muscle-removed bladder implanted with PLCL sheets seeded with hADSCs). Four weeks after the treatment, physiological, histological, immunohistochemical, and immunoblot analyses were performed.
RESULTS
hADSCs were compatible with PLCL sheets. Further, the physiological study of PLCL + ADSC group showed significant improvement in compliance and contractility suggesting the functional improvement of the bladder. Histological, immunohistochemical and immunoblot analyses revealed the uniform distribution of hADSCs in between PLCL sheets as well as differentiation of hADSCs into smooth muscle cells (SMC) which is illustrated by the expression of SMC markers.
CONCLUSION
hADSCs seeded on the multilayered PLCL sheets has the potential to differentiate into SMC, thus facilitating the recovery of compliance and contractility of the injured bladder.
背景
为了研究在去逼尿肌平滑肌的膀胱模型中，种植于多层聚（L-丙交酯-ε-己内酯）（PLCL）片上的人脂肪来源干细胞（hADSCs）是否能改善膀胱功能。
方法
雄性大鼠被随机分为 4 组：正常组、损伤组（去逼尿肌平滑肌的膀胱）、PLCL 组（植入 PLCL 片的去逼尿肌平滑肌的膀胱）和 PLCL+ADSC 组（植入种植有 hADSCs 的 PLCL 片的去逼尿肌平滑肌的膀胱）。治疗 4 周后，进行生理、组织学、免疫组织化学和免疫印迹分析。
结果
hADSCs 与 PLCL 片相容。此外，PLCL+ADSC 组的生理研究显示，顺应性和收缩性有显著改善，表明膀胱功能得到改善。组织学、免疫组织化学和免疫印迹分析显示 hADSCs 均匀分布在 PLCL 片之间，并分化为平滑肌细胞（SMC），这可通过 SMC 标志物的表达来证明。
结论
种植于多层 PLCL 片上的 hADSCs 具有分化为 SMC 的潜力，从而促进受损膀胱顺应性和收缩性的恢复。
Zotero 插件