Prasad A S Arun, Rao K V Bhaskara
J Environ Biol. 2014 May;35(3):549-54.
Bacillus halodurans MTCC 865 was employed for decolorization of textile azo dye, Acid Black-24 (AB-24). Thousand mgl⁻¹ of AB-24 was decolorized with 90% efficiency by the strain within 6 hrs at pH 9 and 37 °C with 5% NaCl under static conditions in screening medium. Decolorization was evaluated by measuring the periodic decrease in absorbance at 557 nm (λ(max)). Biodegradation of Acid Black-24 was determined by FTIR and HPLC. The FTIR spectrum of the AB-24 dye suggests the presence of azo bond (-N = N-) peak at 1618.28 cm⁻¹. Absence of the azo bond in the degraded sample spectrum indicates biodegradation of the dye. Formation of metabolites with different retention times in HPLC analysis further confirmed degradation of the azo dye, Acid Black-24 by Bacillus halodurans.
嗜碱芽孢杆菌MTCC 865用于纺织偶氮染料酸性黑24（AB - 24）的脱色。在筛选培养基中，于pH 9、37℃、含5%氯化钠的静态条件下，该菌株在6小时内可将1000 mgL⁻¹的AB - 24脱色，脱色效率达90%。通过测量557 nm（λ(max)）处吸光度的周期性下降来评估脱色情况。采用傅里叶变换红外光谱（FTIR）和高效液相色谱（HPLC）测定酸性黑24的生物降解情况。AB - 24染料的FTIR光谱表明在1618.28 cm⁻¹处存在偶氮键（-N = N-）峰。降解样品光谱中偶氮键的缺失表明染料发生了生物降解。HPLC分析中不同保留时间代谢物的形成进一步证实了嗜碱芽孢杆菌对偶氮染料酸性黑24的降解。
