Ajaz Mehvish, Rehman Abdul, Khan Zaman, Nisar Muhammad Atif, Hussain Syed
Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore, 54590, Pakistan.
University, Institute of Medical Laboratory Technology (UIMLT), Faculty of Allied Health Sciences (FAHS), The University of Lahore, Lahore, Pakistan.
AMB Express. 2019 May 17;9(1):64. doi: 10.1186/s13568-019-0788-3.
In the present study, Alcaligenes aquatilis was found to decolorize 82% Synazol red 6HBN after incubation of 4 days at 37 °C and pH 7. Maximum decolorization was found under static conditions by using saw dust and yeast extract as carbon and nitrogen source. It also showed promising potential to decolorize mixture of multiple dyes at a rate of more than 86% in 5 days. Decolorization of dye had positive influence on the growth of bacterium as growth rate was increased along with decolorization. The cleavage of azo bond was confirmed through TLC, HPLC and GC-MS analysis. The dye metabolites produced during bacterial treatment are linked to various pathways including ATP synthesis process. The absence of peaks of wavelength 1612/cm and 1532/cm in bacterially treated FTIR sample demonstrated the cleavage of azo bond. Microbial growth in decolorized dye wastewater shows that bacterially decolorized wastewater is unharmful for the growth of micro-flora. The high decolorization ability of A. aquatilis 3c to convert toxic azo dyes into useful end products may find potential applications in the environmental biotechnology.
在本研究中，发现水生产碱菌在37℃和pH 7条件下培养4天后可使82%的西那唑红6HBN脱色。以锯末和酵母提取物作为碳源和氮源，在静态条件下可实现最大脱色效果。它还显示出在5天内以超过86%的速率对多种染料混合物进行脱色的良好潜力。染料脱色对细菌生长有积极影响，因为生长速率随着脱色而增加。通过薄层色谱法（TLC）、高效液相色谱法（HPLC）和气相色谱 - 质谱联用仪（GC - MS）分析证实了偶氮键的断裂。细菌处理过程中产生的染料代谢产物与包括ATP合成过程在内的各种途径相关。经细菌处理的傅里叶变换红外光谱（FTIR）样品中波长1612/cm和1532/cm处无峰，证明了偶氮键的断裂。脱色染料废水中的微生物生长表明，经细菌脱色的废水对微生物群落的生长无害。水生产碱菌3c将有毒偶氮染料转化为有用终产物的高脱色能力可能在环境生物技术中具有潜在应用。
