Babu Eththilu, Muthu Mareeswaran Paulpandian, Singaravadivel Subramanian, Bhuvaneswari Jayaraman, Rajagopal Seenivasan
Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India; Department of Chemistry, VV College of Engineering, Tisaiyanvilai, Tirunelveli, India.
Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India; Graduate School of EEWS (WCU), Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Sep 15;130:553-60. doi: 10.1016/j.saa.2014.04.060. Epub 2014 Apr 20.
A selective, label free luminescence sensor for bovine serum albumin (BSA) is investigated using ruthenium(II) complexes over the other proteins. Interaction between BSA and ruthenium(II) complexes has been studied using absorption, emission, excited state lifetime and circular dichroism (CD) spectral techniques. The luminescence intensity of ruthenium(II) complexes (I and II), has enhanced at 602 and 613 nm with a large hypsochromic shift of 18 and 5 nm respectively upon addition of BSA. The mode of binding of ruthenium(II) complexes with BSA has analyzed using computational docking studies.
研究了一种用于牛血清白蛋白（BSA）的选择性、无标记发光传感器，该传感器使用钌（II）配合物来检测其他蛋白质。已使用吸收、发射、激发态寿命和圆二色性（CD）光谱技术研究了BSA与钌（II）配合物之间的相互作用。加入BSA后，钌（II）配合物（I和II）的发光强度在602和613 nm处增强，分别有18和5 nm的大蓝移。已使用计算对接研究分析了钌（II）配合物与BSA的结合模式。
