基于小分子方酸菁染料在活细胞中的荧光寿命监测牛血清白蛋白的内吞作用。
Monitoring the endocytosis of bovine serum albumin based on the fluorescence lifetime of small squaraine dye in living cells.
作者信息
Lin Fangrui, Das Pintu, Zhao Yihua, Shen Binglin, Hu Rui, Zhou Feifan, Liu Liwei, Qu Junle
机构信息
Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province & Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong Province 518060, China.
出版信息
Biomed Opt Express. 2019 Dec 9;11(1):149-159. doi: 10.1364/BOE.11.000149. eCollection 2020 Jan 1.
Bovine serum albumin (BSA) has a wide range of physiological functions involving the binding, transportation, and delivery of fatty acids, porphyrins, bilirubin, steroids, etc. In the present study, we prepared a small squaraine dye (SD), which can selectively detect BSA using fluorescence lifetime imaging microscopy (FLIM), to monitor the endocytosis of BSA in live cultured cells in real time. This approach revealed that BSA uptake is concentration-dependent in living cells. Furthermore, we used paclitaxel (PTX), a chemotherapeutic drug, to influence the endocytosis of BSA in living cells. The results demonstrated that the endocytic rate was clearly reduced after pretreatment with 0.4 µM PTX for 2 h. The present study demonstrates the potential value of using the fluorescence lifetime of SD to detect BSA concentration and study the physiological mechanism of chemotherapeutic drugs.
牛血清白蛋白（BSA）具有广泛的生理功能，涉及脂肪酸、卟啉、胆红素、类固醇等的结合、运输和递送。在本研究中，我们制备了一种小型方酸菁染料（SD），它可以使用荧光寿命成像显微镜（FLIM）选择性地检测BSA，以实时监测活培养细胞中BSA的内吞作用。该方法表明，活细胞中BSA的摄取具有浓度依赖性。此外，我们使用化疗药物紫杉醇（PTX）来影响活细胞中BSA的内吞作用。结果表明，用0.4 µM PTX预处理2小时后，内吞率明显降低。本研究证明了利用SD的荧光寿命检测BSA浓度以及研究化疗药物生理机制的潜在价值。
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