Halstensen T S, Scott H, Brandtzaeg P
Institute of Pathology, University of Oslo, Rikshospitalet, Norway.
Scand J Immunol. 1989 Dec;30(6):665-72. doi: 10.1111/j.1365-3083.1989.tb02474.x.
Expression of the gamma/delta T-cell receptor (TcR) for antigen on CD3+ intraepithelial lymphocytes (IEL) was studied in situ by two-colour immunofluorescence on jejunal tissue sections from 24 patients with coeliac disease and 17 controls. The proportion of intraepithelial TcR gamma/delta+ cells was significantly increased (P less than 0.002) in untreated (median 20%, range 11-53%) as well as in treated (gluten-free diet) coeliac disease (median 23%, range 16-55%) compared with controls (median 2%, range 0-39%). Although TcR alpha/beta+ IEL dominated both in controls and coeliac disease, T cells expressing the TcR gamma/delta were preferentially located within the epithelium rather than in the lamina propria. Paired staining for TcR gamma/delta and CD8 revealed that most (approximately 90%) intraepithelial TcR gamma/delta+ lymphocytes in coeliac disease were CD8-. A remarkably large fraction (median 67%, range 58-94%) of intraepithelial TcR gamma/delta+ cells expressed the V delta 1/J delta 1-encoded epitope revealed by monoclonal antibody delta TCS1. Our results suggested that increase of the intraepithelial TcR gamma/delta+ CD8- subset of T cells is particularly related to coeliac disease.
通过对24例乳糜泻患者和17例对照者空肠组织切片进行双色免疫荧光原位研究，分析了CD3⁺上皮内淋巴细胞（IEL）上抗原γ/δT细胞受体（TcR）的表达情况。与对照组（中位数2%，范围0 - 39%）相比，未经治疗的乳糜泻患者（中位数20%，范围11 - 53%）以及接受治疗（无麸质饮食）的乳糜泻患者（中位数23%，范围16 - 55%）上皮内TcRγ/δ⁺细胞比例显著增加（P < 0.002）。尽管在对照组和乳糜泻患者中TcRα/β⁺IEL均占主导，但表达TcRγ/δ的T细胞优先位于上皮内而非固有层。对TcRγ/δ和CD8进行双重染色显示，乳糜泻患者中大多数（约90%）上皮内TcRγ/δ⁺淋巴细胞为CD8⁻。上皮内TcRγ/δ⁺细胞中有很大一部分（中位数67%，范围58 - 94%）表达单克隆抗体δTCS1所识别的Vδ1/Jδ1编码表位。我们的结果表明，上皮内TcRγ/δ⁺CD8⁻T细胞亚群的增加与乳糜泻尤其相关。
