应用磁性固相萃取高效液相色谱-荧光检测法分离测定谷物产品中黄曲霉毒素 B₁ 和 B₂。
Application of magnetic solid phase extraction for separation and determination of aflatoxins B ₁ and B₂ in cereal products by high performance liquid chromatography-fluorescence detection.
机构信息
Department of Analytical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran.
Department of Analytical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jun 1;960:200-8. doi: 10.1016/j.jchromb.2014.03.035. Epub 2014 Apr 5.
A simple and sensitive method based on the magnetic solid phase extraction with modified magnetic nanoparticles followed by high performance liquid chromatography with fluorescence detection has been developed for extraction and determination of aflatoxins B1 (AFB1) and B2 (AFB2) in cereal products. Magnetic nanoparticle coated with 3-(trimethoxysilyl)-1-propanthiol (TMSPT) and modified with 2-amino-5-mercapto-1,3,4-thiadiazole (AMT) was used as an antibody-free adsorbent. Under the optimal conditions, the calibration curves for AFB1 and AFB2 were linear in the ranges of 0.2-15 μg L(-1) and 0.04-3 μg L(-1), respectively. Detection limit was 0.041 μg L(-1) for AFB1 and 0.013 μg L(-1) for AFB2. The proposed method was successfully applied to the determination of AFB1 and AFB2 in spiked corn and rice samples with an average recovery of 93.5%. The results demonstrated that the developed method is simple, rapid, inexpensive, accurate and remarkably free from interference effects.
基于磁性固相萃取与修饰后的磁性纳米粒子结合高效液相色谱荧光检测，建立了一种用于谷物制品中黄曲霉毒素 B1（AFB1）和 B2（AFB2）提取和测定的简单、灵敏的方法。经 3-（三甲氧基硅基）-1-丙硫醇（TMSPT）修饰并修饰 2-氨基-5-巯基-1,3,4-噻二唑（AMT）的磁性纳米粒子被用作无抗体吸附剂。在最佳条件下，AFB1 和 AFB2 的校准曲线在 0.2-15 μg L(-1)和 0.04-3 μg L(-1)范围内呈线性。AFB1 的检测限为 0.041 μg L(-1)，AFB2 的检测限为 0.013 μg L(-1)。该方法成功应用于加标玉米和大米样品中 AFB1 和 AFB2 的测定，平均回收率为 93.5%。结果表明，该方法简单、快速、廉价、准确，且干扰效应显著降低。
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