Li Qing, Jiang Luyi, Zhang Huan, Wei Mengmeng, Chu Chu, Yan Jizhong
Zhejiang University of Technology, College of Pharmaceutical Science, Zunyi W Rd, Hangzhou 310058, China.
J AOAC Int. 2019 May 1;102(3):761-766. doi: 10.5740/jaoacint.18-0311. Epub 2018 Nov 2.
Aflatoxins (AFs) have attracted increasing amounts of attention in recent years for their high toxicity. Previously, AF-polluted Chinese Patent Medicines (CPMs) were ignored, but their quality, safety, and efficacy might be influenced by the toxic compounds. The objective is to develop a simple, low-cost, and efficient method for quantitative analysis of aflatoxins B (AFB) and B₂ (AFB₂) in CPMs to ensure their safe use. A simple, modified, magnetic nanoparticle-based solid-phase extraction (SPE) combined with HPLC-fluorescence detection for the determination of trace amounts of AFB and AFB₂ was established. The main parameters affecting the efficiency of modified magnetic nanoparticle-based SPE, such as pH of sample solution, adsorbent amount, adsorption time, and desorption condition, were investigated. Under the optimum conditions, AFB and AFB₂ were linear in the ranges of 0.3-10 and 0.04-3 ng/mL with the correlation coefficient () of 0.9998 and 0.9999, respectively. Their intraday precisions were 1.16 and 2.30% and the interday precisions were 1.28 and 1.87% for AFB and AFB₂, respectively. The developed SPE was applied for AFB and AFB₂ extraction in three commercially available Fupuganmao granule samples, and the results were compared with the official immunoaffinity column chromatography method. The method provided a preferable candidate for the determination approach of AF measurement in CPMs. Amine-functionalized magnetic nanoparticles were successfully applied to SPE for adsorbing AFB and AFB₂ in CPMs for the first time.
黄曲霉毒素（AFs）近年来因其高毒性而受到越来越多的关注。此前，受AF污染的中成药（CPMs）被忽视，但其质量、安全性和有效性可能会受到这些有毒化合物的影响。目的是开发一种简单、低成本且高效的方法，用于定量分析中成药中的黄曲霉毒素B（AFB）和B₂（AFB₂），以确保其安全使用。建立了一种简单的、基于磁性纳米颗粒的改良固相萃取（SPE）结合高效液相色谱 - 荧光检测法，用于测定痕量的AFB和AFB₂。研究了影响基于磁性纳米颗粒的改良SPE效率的主要参数，如样品溶液的pH值、吸附剂用量、吸附时间和解吸条件。在最佳条件下，AFB和AFB₂在0.3 - 10和0.04 - 3 ng/mL范围内呈线性，相关系数（）分别为0.9998和0.9999。AFB和AFB₂的日内精密度分别为1.16%和2.30%，日间精密度分别为1.28%和1.87%。所开发的SPE应用于三种市售的感冒清热颗粒样品中AFB和AFB₂的提取，并将结果与官方免疫亲和柱色谱法进行比较。该方法为中成药中AF测定的方法提供了一个较好的选择。胺功能化磁性纳米颗粒首次成功应用于SPE，用于吸附中成药中的AFB和AFB₂。
