依达拉奉是一种自由基清除剂，可提高猪非心脏跳动供体肝脏移植的移植物存活率。
Edaravone, a free radical scavenger, improves the graft viability on liver transplantation from non-heart-beating donors in pigs.
作者信息
Miyazawa K, Miyagi S, Maida K, Murakami K, Fujio A, Kashiwadate T, Nakanishi W, Hara Y, Nakanishi C, Yamaya H, Kawagishi N, Goto M, Ohuchi N
机构信息
Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
出版信息
Transplant Proc. 2014 May;46(4):1090-4. doi: 10.1016/j.transproceed.2013.11.155.
BACKGROUND
Although liver transplantation from non-heart-beating donors (NHBDs) is an effective way to overcome shortage of donors, primary graft nonfunction is often noted in these grafts. We have previously reported that edaravone, a free radical scavenger, has a cytoprotective effect on warm ischemia-reperfusion injury and improves the function of liver grafts from NHBDs in a rat model of ischemia-reperfusion. The purpose of this study was to investigate the effects of edaravone on liver transplantations from NHBDs.
METHODS
Pigs were divided into three groups: (1) a heart-beating (HB) group (n = 5), in which liver grafts were retrieved from HB donors; (2) a non-heart-beating (NHB) group (n = 4), in which liver grafts were retrieved under apnea-induced NHB conditions; and (3) an edaravone-treated (ED) group (n = 5), in which liver grafts were retrieved in the same manner as the NHB group and treated with edaravone at the time of perfusion (3 mg/L in University of Wisconsin [UW] solution), cold preservation (1 mg/L in UW solution), and after surgery (1 mg/kg/d). The grafts from all groups were transplanted after 4 hours of cold preservation.
RESULTS
In the ED group, the 7-day survival rate was significantly higher than that in the NHB group (80% versus 0%, P = .0042, Kaplan-Meier log-rank test). Furthermore, on histologic examination, the structure of sinusoids in the ED group was well preserved and similar to that in the HB group.
CONCLUSIONS
Edaravone may improve the viability of liver grafts from NHBDs.
背景
尽管非心脏跳动供体（NHBDs）肝移植是克服供体短缺的有效方法，但这些移植物中常出现原发性移植物无功能。我们之前报道过，自由基清除剂依达拉奉对热缺血-再灌注损伤具有细胞保护作用，并在缺血-再灌注大鼠模型中改善了NHBDs肝移植物的功能。本研究的目的是探讨依达拉奉对NHBDs肝移植的影响。
方法
将猪分为三组：（1）心脏跳动（HB）组（n = 5），其肝移植物取自HB供体；（2）非心脏跳动（NHB）组（n = 4），其肝移植物在呼吸暂停诱导的NHB条件下获取；（3）依达拉奉治疗（ED）组（n = 5），其肝移植物以与NHB组相同的方式获取，并在灌注时（在威斯康星大学[UW]溶液中为3 mg/L）、冷保存时（在UW溶液中为1 mg/L）和术后（1 mg/kg/d）用依达拉奉治疗。所有组的移植物在冷保存4小时后进行移植。
结果
在ED组中，7天生存率显著高于NHB组（80%对0%，P = .0042，Kaplan-Meier对数秩检验）。此外，组织学检查显示，ED组肝血窦结构保存良好，与HB组相似。
结论
依达拉奉可能提高NHBDs肝移植物的存活率。
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