一种自由基清除剂依达拉奉可预防非心脏骤停供体肝脏移植中的缺血再灌注损伤。
A free radical scavenger, edaravone, prevents ischemia-reperfusion injury in liver grafts from non-heart-beating donors.
作者信息
Nakamura A, Akamatsu Y, Miyagi S, Fukumori T, Sekiguchi S, Satomi S
机构信息
Division of Advanced Surgical Science and Technology, Tohoku University Graduate School of Medicine, Sendai, Japan.
出版信息
Transplant Proc. 2008 Sep;40(7):2171-4. doi: 10.1016/j.transproceed.2008.06.049.
BACKGROUND
Due to the increase in liver transplantation, the donor shortage has become a serious problem, requiring marginal, non-heart-beating donors (NHBDs). The aims of this study were to evaluate the cytoprotective effect of edaravone, a free radical scavenger, on warm ischemia-reperfusion (I/R) injury of liver grafts from NHBDs.
METHODS
Rat livers were harvested from heart-beating donors (HB group) or from NHBDs undergoing cardiac arrest for 30 minutes led by thoracotomy (NHB group), and reperfused for 60 minutes with Krebs-Henseleit bicarbonate buffer after cold preservation for 6 hours. In another group (ED group), warm ischemic livers from NHBDs were reperfused with buffer containing edaravone (1 mg/L) after cold preservation.
RESULTS
In the ED group, portal flow volume, bile production, and energy charge were significantly ameliorated. Lipid peroxidation, elevation of hepatic enzymes, and release of tumor necrosis factor-alpha and interleukin-1 beta were significantly alleviated, compared with the NHB group.
CONCLUSIONS
These results suggested that edaravone has suppressive effects on warm I/R injury in liver grafts from NHBDs.
背景
由于肝移植数量的增加，供体短缺已成为一个严重问题，需要使用边缘性非心脏跳动供体（NHBDs）。本研究的目的是评估自由基清除剂依达拉奉对NHBDs肝脏移植物热缺血再灌注（I/R）损伤的细胞保护作用。
方法
从心跳供体（HB组）或通过开胸诱导心脏骤停30分钟的NHBDs获取大鼠肝脏（NHB组），在冷保存6小时后用Krebs-Henseleit碳酸氢盐缓冲液再灌注60分钟。在另一组（ED组）中，NHBDs的热缺血肝脏在冷保存后用含依达拉奉（1mg/L）的缓冲液再灌注。
结果
在ED组中，门静脉血流量、胆汁生成和能量电荷显著改善。与NHB组相比，脂质过氧化、肝酶升高以及肿瘤坏死因子-α和白细胞介素-1β的释放显著减轻。
结论
这些结果表明依达拉奉对NHBDs肝脏移植物的热I/R损伤具有抑制作用。
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