Nakamura A, Akamatsu Y, Miyagi S, Fukumori T, Sekiguchi S, Satomi S
Division of Advanced Surgical Science and Technology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Transplant Proc. 2008 Sep;40(7):2171-4. doi: 10.1016/j.transproceed.2008.06.049.
Due to the increase in liver transplantation, the donor shortage has become a serious problem, requiring marginal, non-heart-beating donors (NHBDs). The aims of this study were to evaluate the cytoprotective effect of edaravone, a free radical scavenger, on warm ischemia-reperfusion (I/R) injury of liver grafts from NHBDs.
Rat livers were harvested from heart-beating donors (HB group) or from NHBDs undergoing cardiac arrest for 30 minutes led by thoracotomy (NHB group), and reperfused for 60 minutes with Krebs-Henseleit bicarbonate buffer after cold preservation for 6 hours. In another group (ED group), warm ischemic livers from NHBDs were reperfused with buffer containing edaravone (1 mg/L) after cold preservation.
In the ED group, portal flow volume, bile production, and energy charge were significantly ameliorated. Lipid peroxidation, elevation of hepatic enzymes, and release of tumor necrosis factor-alpha and interleukin-1 beta were significantly alleviated, compared with the NHB group.
These results suggested that edaravone has suppressive effects on warm I/R injury in liver grafts from NHBDs.
由于肝移植数量的增加，供体短缺已成为一个严重问题，需要使用边缘性非心脏跳动供体（NHBDs）。本研究的目的是评估自由基清除剂依达拉奉对NHBDs肝脏移植物热缺血再灌注（I/R）损伤的细胞保护作用。
从心跳供体（HB组）或通过开胸诱导心脏骤停30分钟的NHBDs获取大鼠肝脏（NHB组），在冷保存6小时后用Krebs-Henseleit碳酸氢盐缓冲液再灌注60分钟。在另一组（ED组）中，NHBDs的热缺血肝脏在冷保存后用含依达拉奉（1mg/L）的缓冲液再灌注。
在ED组中，门静脉血流量、胆汁生成和能量电荷显著改善。与NHB组相比，脂质过氧化、肝酶升高以及肿瘤坏死因子-α和白细胞介素-1β的释放显著减轻。
这些结果表明依达拉奉对NHBDs肝脏移植物的热I/R损伤具有抑制作用。
