Department of Food Materials and Process Design Engineering, Faculty of Food Science and Technology, University of Agricultural Sciences and Natural Resources, Beheshti Avenue, Gorgan, Iran.
Department of Food Science and Technology, Faculty of Agriculture Engineering and Technology, College of Agriculture and Natural Resource, University of Tehran, Karaj, Iran.
Carbohydr Polym. 2014 Aug 30;109:148-54. doi: 10.1016/j.carbpol.2014.03.063. Epub 2014 Apr 1.
Chitosan-nanocellulose biocomposites were prepared from chitosan having molecular weight of 600-800 kDa, nanocellulose with 20-50 nm diameters and various levels of 30, 60 and 90% (v/wCHT) for glycerol. Agitation and sonication were used to facilitate even dispersion of particles in the polymer matrix. The nanocomposites were examined by differential scanning calorimetry, X-ray diffraction and agar disc diffusion tests; finally, the film was applied on the surface of ground meat to evaluate its performance in real terms. Chitosan-nanocellulose nanocomposites showed high Tg range of 115-124°C and were able to keep their solid state until the temperature (Tm) range of 97-99°C. XRD photographs revealed that nanocellulose peak completely disappeared after their addition to chitosan context. Agar disc diffusion method proved that the nancomposite had inhibitory effects against both gram-positive (S. aureus) and gram-negative (E. coli and S. enteritidis) bacteria through its contact area. Application of chitosan-nanocellulose nanocomposite on the ground meat decreased lactic acid bacteria population compared with nylon packaged samples up to 1.3 and 3.1 logarithmic cycles at 3 and 25°C after 6 days of storage, respectively.
壳聚糖-纳米纤维素生物复合材料由分子量为 600-800 kDa 的壳聚糖、直径为 20-50nm 的纳米纤维素以及 30%、60%和 90%（v/wCHT）的不同甘油水平制备而成。搅拌和超声处理用于促进颗粒在聚合物基质中的均匀分散。通过差示扫描量热法、X 射线衍射和琼脂盘扩散试验对纳米复合材料进行了研究；最后，将薄膜应用于碎肉表面，以实际评估其性能。壳聚糖-纳米纤维素纳米复合材料具有 115-124°C 的高玻璃化转变温度范围，并且能够保持其固态状态，直到 97-99°C 的温度（Tm）范围。X 射线衍射照片表明，纳米纤维素峰在添加到壳聚糖环境后完全消失。琼脂盘扩散法证明，纳米复合材料通过其接触面积对革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌和肠炎沙门氏菌）细菌均具有抑制作用。与尼龙包装的样品相比，壳聚糖-纳米纤维素纳米复合材料在 3 和 25°C 下储存 6 天后，分别使碎肉中的乳酸菌数量减少了 1.3 和 3.1 个对数周期。
