Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China.
Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Department of Central Laboratory, Central Hospital of Wuhan, Wuhan 430014, China; Cancer Research Institute of Wuhan, Wuhan 430014, China.
FEBS Lett. 2014 Jun 5;588(12):2107-14. doi: 10.1016/j.febslet.2014.04.044. Epub 2014 May 8.
Various studies have reported that Huaier possesses anti-tumor effects. However, the mechanisms are not completely elucidated. Here, we found 66 differentially expressed miRNAs in Huaier-treated pulmonary adenocarcinoma A549 cells, with upregulation of miR-26b-5p. Transfection of A549 cells with miR-26b-5p mimic inhibited proliferation and induced apoptosis, while transfection of Huaier-treated A549 cells with a miR-26b-5p inhibitor reversed the effects of Huaier. EZH2 was verified as the target of miR-26b-5p. Thus, our findings indicate that Huaier might suppress proliferation and induce apoptosis in lung cancer cells via a miR-26b-5p-EZH2-mediated approach, which provides a new perspective for understanding the anti-tumor effects of Huaier.
已有多项研究报道，香菇多糖具有抗肿瘤作用。但其具体机制尚未完全阐明。本研究发现香菇多糖处理后的肺腺癌细胞 A549 中有 66 个差异表达的 miRNA，其中 miR-26b-5p 呈上调表达。用 miR-26b-5p 模拟物转染 A549 细胞可抑制增殖并诱导细胞凋亡，而用 miR-26b-5p 抑制剂转染经香菇多糖处理的 A549 细胞则可逆转香菇多糖的作用。EZH2 被验证为 miR-26b-5p 的靶基因。因此，我们的研究结果表明，香菇多糖可能通过 miR-26b-5p-EZH2 介导的途径抑制肺癌细胞增殖并诱导其凋亡，为理解香菇多糖的抗肿瘤作用提供了新视角。
