Department of Dermatology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Acta Histochem. 2013 Sep;115(7):705-11. doi: 10.1016/j.acthis.2013.02.010. Epub 2013 Apr 16.
In recent years, an aqueous extract of the fungus Trametes robiniophila Murriell 1907 (Huaier) has been commonly used in China for complementary cancer therapy. However, the mechanisms of its anticancer effects are largely unknown. In the present study, we aimed to investigate the effects of Huaier extract on the inhibition of proliferation and promotion of apoptosis in a melanoma cell line, A875, and to explore the possible mechanisms of its anticancer effects. Cell proliferation was measured using a Cell Counting Kit-8 (CCK8) and PCNA-Western blot. The cell cycle distribution, and apoptosis levels were analyzed by flow cytometry, and Western blot was used to test the apoptotic pathways. We found that Huaier extract strongly inhibited cell proliferation of the A875 melanoma cells and induced G2/M arrest and apoptosis in a time- and dose-dependent manner. P53 expression was increased and cell apoptosis executed by caspase-3. Down-regulation of Bcl-2 and up-regulation of Bcl2-associated X protein (BAX) indicated that Huaier extract induced apoptosis through the mitochondrial pathway. As expected, the inhibitor Huaier decreased melanoma cell line A875 proliferation, and induced apoptosis in a time- and dose-dependent manner. Our findings indicate that Huaier extract is an effective complementary agent for cancer treatment of melanoma.
近年来，从真菌槐栓菌（Trametes robiniophila Murriell 1907）中提取的水溶性提取物已在中国广泛用于癌症的辅助治疗。然而，其抗癌作用的机制在很大程度上尚不清楚。在本研究中，我们旨在研究槐耳提取物对黑色素瘤细胞系 A875 增殖抑制和促进凋亡的影响，并探讨其抗癌作用的可能机制。使用细胞计数试剂盒-8（CCK8）和 PCNA-Western blot 检测细胞增殖。通过流式细胞术分析细胞周期分布和凋亡水平，Western blot 检测凋亡途径。我们发现槐耳提取物可强烈抑制 A875 黑色素瘤细胞的增殖，并呈时间和剂量依赖性诱导 G2/M 期阻滞和凋亡。p53 表达增加，caspase-3 执行细胞凋亡。Bcl-2 下调和 Bcl2 相关 X 蛋白（BAX）上调表明槐耳提取物通过线粒体途径诱导细胞凋亡。正如预期的那样，抑制剂槐耳可降低黑色素瘤细胞系 A875 的增殖，并呈时间和剂量依赖性诱导细胞凋亡。我们的研究结果表明槐耳提取物是治疗黑色素瘤的有效辅助药物。
