Rao A J, Chakraborti R
Department of Biochemistry, Indian Institute of Science, Bangalore.
J Immunol Methods. 1989 Dec 20;125(1-2):261-4. doi: 10.1016/0022-1759(89)90101-4.
A study has been carried out on the non-specific interference due to serum in the avidin biotin microELISA for monkey chorionic gonadotropin. Results suggest that it is not due to any proteolytic activity in the serum, but immunoglobulin or associated factors interfering at the level of antigen-antibody interaction. This interference was eliminated by heating samples at 60 degrees C for 30 min.
针对用于检测猴绒毛膜促性腺激素的抗生物素蛋白-生物素微酶联免疫吸附测定中血清引起的非特异性干扰进行了一项研究。结果表明，这并非由于血清中的任何蛋白水解活性，而是免疫球蛋白或相关因子在抗原-抗体相互作用水平上产生干扰。通过将样品在60℃加热30分钟可消除这种干扰。
