Wang Yue-Ming, Liu Xiao-Yu, Jiang Cong-Li, Huang Li-Nian, Sun Xin, Liu Zhi-Gang
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2013 Dec;31(6):490-2.
Fifty living mites (Dermatophagoides farinae) were fixed in 2.5% glutaraldehyde, postfixed in 1% osmium tetroxide, dehydrated in a graded ethanol series, embedded in embedding medium. The ultrastructure of the digestive tract in D. farinae was observed by serial ultrathin sections with a transmission electron microscope. The alimentary canal of D. farinae consists of the cuticle-lined foregut and hindgut separated by a microvilli-lined midgut (anterior midgut, posterior midgut). There are different types of epithelial cells in the anterior midgut The microvilli of epithelial cells in posterior midgut are longer than that of the anterior midgut In posterior midgut, the food bolus is surrounded by the peritrophic membrane. The midgut is the main site of digestion and absorption.
五十只活螨(粉尘螨)用2.5%戊二醛固定,再用1%四氧化锇后固定,经梯度乙醇系列脱水,然后包埋于包埋介质中。通过透射电子显微镜对粉尘螨消化道的超微结构进行连续超薄切片观察。粉尘螨的消化道由角质层内衬的前肠和后肠组成,中间被微绒毛内衬的中肠(前中肠、后中肠)隔开。前中肠有不同类型的上皮细胞。后中肠上皮细胞的微绒毛比前中肠的长。在后中肠,食团被围食膜包围。中肠是消化和吸收的主要部位。