Gómez-Lozano María, Marvig Rasmus Lykke, Molin Søren, Long Katherine S
Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
Methods Mol Biol. 2014;1149:433-56. doi: 10.1007/978-1-4939-0473-0_34.
Small regulatory RNAs (sRNAs) in bacteria are known to modulate gene expression and control a variety of processes including metabolic reactions, stress responses, and pathogenesis in response to environmental signals. A method to identify bacterial sRNAs on a genome-wide scale based on RNA sequencing (RNA-seq) is described that involves the preparation and analysis of three different sequencing libraries. As a significant number of unique sRNAs are identified in each library, the libraries can be used either alone or in combination to increase the number of sRNAs identified. The approach may be applied to identify sRNAs in any bacterium under different growth and stress conditions.
已知细菌中的小调控RNA（sRNA）可调节基因表达，并控制各种过程，包括代谢反应、应激反应以及响应环境信号的致病过程。本文描述了一种基于RNA测序（RNA-seq）在全基因组范围内鉴定细菌sRNA的方法，该方法涉及制备和分析三种不同的测序文库。由于在每个文库中都鉴定出了大量独特的sRNA，这些文库可以单独使用或组合使用，以增加鉴定出的sRNA数量。该方法可用于在不同生长和应激条件下鉴定任何细菌中的sRNA。
