铜绿假单胞菌感染秀丽隐杆线虫的转录分析。
Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans.
作者信息
MacKenzie Ashleigh, Stewart Lewis, Hoskisson Paul A, Tucker Nicholas P
机构信息
The James Hutton Institute, Invergowrie, Dundee, UK.
出版信息
Methods Mol Biol. 2014;1149:607-12. doi: 10.1007/978-1-4939-0473-0_46.
PMID:24818936
Abstract
Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.
摘要
在此，我们描述了一种从感染铜绿假单胞菌的秀丽隐杆线虫中提取总RNA的方法。该方法可排除未被线虫摄取的铜绿假单胞菌细胞，并产生可用于检测宿主和病原体转录本的总RNA。
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