Liu C P, Fu J, Lin S L, Wang X S, Li S
Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China,
Biol Trace Elem Res. 2014 Jun;159(1-3):192-8. doi: 10.1007/s12011-014-0005-9. Epub 2014 May 13.
Selenium (Se) is an essential trace element in many life forms due to its occurrence as selenocysteine (Sec) residue in selenoproteins. However, little is known about the expression pattern of selenoproteins in the liver of layer chicken. To investigate the effects of Se deficiency on the mRNA expressions of selenoproteins in the liver tissue of layer chickens, 1-day-old layer chickens were randomly allocated into two groups (n=120/group). The Se-deficient group (-Se) was fed a Se-deficient corn-soy basal diet; the Se-adequate group as control (+Se) was fed the same basal diet supplemented with Se at 0.15 mg/kg (sodium selenite). The liver tissue was collected and examined for mRNA levels of 21 selenoprotein genes at 15, 25, 35, 45, 55, and 65 days old. The data indicated that the mRNA expressions of Gpx1, Gpx2, Gpx3, Gpx4, Sepn1, Sepp1, Selo, Sepx1, Selu, Txnrd1, Txnrd2, Txnrd3, Dio1, Dio2, SPS2, Selm, SelPb, Sep15, and Sels were decreased (p<0.05), but not the levels of Dio3 and Seli (p>0.05). The results showed that the mRNA levels of 19 selenoprotein (except Seli and Dio3) genes in the layer chicken liver were regulated by diet Se level. The present study provided some compensated data about the roles of Se in the regulation of selenoproteins.
硒（Se）是许多生命形式中必需的微量元素，因为它以硒代半胱氨酸（Sec）残基的形式存在于硒蛋白中。然而，关于蛋鸡肝脏中硒蛋白的表达模式知之甚少。为了研究缺硒对蛋鸡肝脏组织中硒蛋白mRNA表达的影响，将1日龄蛋鸡随机分为两组（每组n = 120）。缺硒组（-Se）饲喂缺硒的玉米-大豆基础日粮；作为对照的富硒组（+Se）饲喂添加了0.15 mg/kg硒（亚硒酸钠）的相同基础日粮。在15、25、35、45、55和65日龄时收集肝脏组织，检测21种硒蛋白基因的mRNA水平。数据表明，Gpx1、Gpx2、Gpx3、Gpx4、Sepn1、Sepp1、Selo、Sepx1、Selu、Txnrd1、Txnrd2、Txnrd3、Dio1、Dio2、SPS2、Selm、SelPb、Sep15和Sels的mRNA表达降低（p<0.05），但Dio3和Seli的水平未降低（p>0.05）。结果表明，蛋鸡肝脏中19种硒蛋白（除Seli和Dio3外）基因的mRNA水平受日粮硒水平的调节。本研究提供了一些关于硒在硒蛋白调节中作用的补充数据。
