Jalali Seyed Sattar, Talebi Jalali, Allymehr Manoochehr, Soleimanzadeh Ali, Razi Mazdak
Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2019 Spring;10(2):139-144. doi: 10.30466/vrf.2018.86992.2128. Epub 2019 Jun 15.
Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in the fertility of eggs in broiler breeder farms. Todays, supplementation of chicken diet with additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects of different levels of nano-selenium (Nano-Se) on the expression of molecular markers of spermatogonial stem cells (SSCs) in the testis of broiler breeder males. A total of 30 roosters of 40 weeks of age were randomly divided into five groups. Groups were as follows: 1) control (normal diet) group, 2) diet supplemented with 0.30 mg kg sodium selenite, 3) diet supplemented with 0.15 mg kg Nano-Se, 4) diet supplemented with 0.30 mg kg Nano-Se, and 5) diet supplemented with 0.60 mg kg Nano-Se. At the end of the experimental period (5 week), birds were autopsied and samples from testis of all birds were collected. The testis samples were used to examine the β1-integrin (CD29), thy-1 (CD90) and NANOG mRNA expression by real-time PCR. The results showed that testis of the groups fed with the diets supplemented with 0.60 mg kg and 0.15 mg kg of Nano-Se had the highest and lowest mRNA expression of SSCs markers, respectively. In conclusion, the present study indicated that Nano-Se had advantages over sodium selenite. Diet supplemented with 0.60 mg kg of Nano-Se may contribute to optimal fertility via increasing the mRNA expression of SSCs markers of roosters' testis and could be used to delay the reduction of fertility caused by aging in broiler breeder males.
繁殖力是种鸡场最重要的参数之一，公鸡在肉鸡种鸡场的种蛋受精方面发挥着重要作用。如今，在鸡的日粮中添加有机硒等添加剂可用于提高繁殖力。本研究的目的是评估不同水平的纳米硒（Nano-Se）对肉种鸡雄性睾丸中精原干细胞（SSCs）分子标志物表达的影响。总共30只40周龄的公鸡被随机分为五组。分组如下：1）对照组（正常日粮），2）日粮添加0.30 mg/kg亚硒酸钠，3）日粮添加0.15 mg/kg纳米硒，4）日粮添加0.30 mg/kg纳米硒，5）日粮添加0.60 mg/kg纳米硒。在实验期结束时（5周），对鸡进行解剖，并收集所有鸡的睾丸样本。睾丸样本用于通过实时PCR检测β1整合素（CD29）、thy-1（CD90）和NANOG mRNA的表达。结果表明，日粮添加0.60 mg/kg和0.15 mg/kg纳米硒的组中，睾丸中SSCs标志物的mRNA表达分别最高和最低。总之，本研究表明纳米硒优于亚硒酸钠。日粮添加0.60 mg/kg纳米硒可能通过增加公鸡睾丸中SSCs标志物的mRNA表达来促进最佳繁殖力，并可用于延缓肉种鸡雄性因衰老导致的繁殖力下降。
