lptA的相变表达调节淋病奈瑟菌对阳离子抗菌肽的抗性。
Phase-variable expression of lptA modulates the resistance of Neisseria gonorrhoeae to cationic antimicrobial peptides.
作者信息
Kandler Justin L, Joseph Sandeep J, Balthazar Jacqueline T, Dhulipala Vijaya, Read Timothy D, Jerse Ann E, Shafer William M
机构信息
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA.
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
出版信息
Antimicrob Agents Chemother. 2014 Jul;58(7):4230-3. doi: 10.1128/AAC.03108-14. Epub 2014 May 12.
Abstract
Phosphoethanolamine (PEA) decoration of lipid A produced by Neisseria gonorrhoeae has been linked to bacterial resistance to cationic antimicrobial peptides/proteins (CAMPs) and in vivo fitness during experimental infection. We now report that the lptA gene, which encodes the PEA transferase responsible for this decoration, is in an operon and that high-frequency mutation in a polynucleotide repeat within lptA can influence gonococcal resistance to CAMPs.
摘要
淋病奈瑟菌产生的脂多糖A上的磷酸乙醇胺（PEA）修饰与细菌对阳离子抗菌肽/蛋白质（CAMP）的抗性以及实验感染期间的体内适应性有关。我们现在报告，编码负责这种修饰的PEA转移酶的lptA基因位于一个操纵子中，并且lptA内多核苷酸重复序列中的高频突变会影响淋球菌对CAMP的抗性。
相似文献
1
Phase-variable expression of lptA modulates the resistance of Neisseria gonorrhoeae to cationic antimicrobial peptides.
Antimicrob Agents Chemother. 2014 Jul;58(7):4230-3. doi: 10.1128/AAC.03108-14. Epub 2014 May 12.
2
Phosphoethanolamine decoration of Neisseria gonorrhoeae lipid A plays a dual immunostimulatory and protective role during experimental genital tract infection.
Infect Immun. 2014 Jun;82(6):2170-9. doi: 10.1128/IAI.01504-14. Epub 2014 Mar 31.
3
Phosphoethanolamine substitution of lipid A and resistance of Neisseria gonorrhoeae to cationic antimicrobial peptides and complement-mediated killing by normal human serum.
Infect Immun. 2009 Mar;77(3):1112-20. doi: 10.1128/IAI.01280-08. Epub 2008 Dec 29.
4
Lipid A's structure mediates Neisseria gonorrhoeae fitness during experimental infection of mice and men.
mBio. 2013 Nov 19;4(6):e00892-13. doi: 10.1128/mBio.00892-13.
5
Cationic antimicrobial peptide resistance in Neisseria meningitidis.
J Bacteriol. 2005 Aug;187(15):5387-96. doi: 10.1128/JB.187.15.5387-5396.2005.
6
Phosphoethanolamine Transferase LptA in Haemophilus ducreyi Modifies Lipid A and Contributes to Human Defensin Resistance In Vitro.
PLoS One. 2015 Apr 22;10(4):e0124373. doi: 10.1371/journal.pone.0124373. eCollection 2015.
7
Novel small molecules that increase the susceptibility of Neisseria gonorrhoeae to cationic antimicrobial peptides by inhibiting lipid A phosphoethanolamine transferase.
J Antimicrob Chemother. 2022 Aug 25;77(9):2441-2447. doi: 10.1093/jac/dkac204.
8
The MisR Response Regulator Is Necessary for Intrinsic Cationic Antimicrobial Peptide and Aminoglycoside Resistance in Neisseria gonorrhoeae.
Antimicrob Agents Chemother. 2016 Jul 22;60(8):4690-700. doi: 10.1128/AAC.00823-16. Print 2016 Aug.
9
The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin.
J Mol Biol. 2013 Sep 23;425(18):3389-402. doi: 10.1016/j.jmb.2013.06.029. Epub 2013 Jun 28.
10
Phosphoethanolamine Modification of Neisseria gonorrhoeae Lipid A Reduces Autophagy Flux in Macrophages.
PLoS One. 2015 Dec 7;10(12):e0144347. doi: 10.1371/journal.pone.0144347. eCollection 2015.
引用本文的文献
1
Advances in the Role of Antimicrobial Peptides in the Management of Sexually Transmitted Infections.
J Clin Lab Anal. 2025 Jun;39(11):e70041. doi: 10.1002/jcla.70041. Epub 2025 May 14.
2
Determinants of bacterial survival and proliferation in blood.
FEMS Microbiol Rev. 2024 May 8;48(3). doi: 10.1093/femsre/fuae013.
3
Polymyxin Stereochemistry and Its Role in Antibacterial Activity and Outer Membrane Disruption.
ACS Infect Dis. 2022 Dec 9;8(12):2396-2404. doi: 10.1021/acsinfecdis.2c00307. Epub 2022 Nov 7.
4
Novel small molecules that increase the susceptibility of Neisseria gonorrhoeae to cationic antimicrobial peptides by inhibiting lipid A phosphoethanolamine transferase.
J Antimicrob Chemother. 2022 Aug 25;77(9):2441-2447. doi: 10.1093/jac/dkac204.
5
Anti-Virulence Therapeutic Approaches for .
Antibiotics (Basel). 2021 Jan 21;10(2):103. doi: 10.3390/antibiotics10020103.
6
Predominant phosphorylation patterns in lipid A determined by top-down MS/MS.
J Lipid Res. 2020 Nov;61(11):1437-1449. doi: 10.1194/jlr.RA120001014. Epub 2020 Aug 24.
7
Efficacy of Antigonococcal CMP-Nonulosonate Therapeutics Require Cathelicidins.
J Infect Dis. 2020 Oct 13;222(10):1641-1650. doi: 10.1093/infdis/jiaa438.
8
Identification of a Histone Deacetylase: Epigenetic Impact on Host Gene Expression.
Pathogens. 2020 Feb 18;9(2):132. doi: 10.3390/pathogens9020132.
9
Structure-Function Relationships of the Neisserial EptA Enzyme Responsible for Phosphoethanolamine Decoration of Lipid A: Rationale for Drug Targeting.
Front Microbiol. 2018 Aug 21;9:1922. doi: 10.3389/fmicb.2018.01922. eCollection 2018.
10
Gonococcal Defenses against Antimicrobial Activities of Neutrophils.
Trends Microbiol. 2018 Dec;26(12):1022-1034. doi: 10.1016/j.tim.2018.07.003. Epub 2018 Aug 13.
本文引用的文献
1
Phosphoethanolamine decoration of Neisseria gonorrhoeae lipid A plays a dual immunostimulatory and protective role during experimental genital tract infection.
Infect Immun. 2014 Jun;82(6):2170-9. doi: 10.1128/IAI.01504-14. Epub 2014 Mar 31.
2
Lipid A's structure mediates Neisseria gonorrhoeae fitness during experimental infection of mice and men.
mBio. 2013 Nov 19;4(6):e00892-13. doi: 10.1128/mBio.00892-13.
3
The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin.
J Mol Biol. 2013 Sep 23;425(18):3389-402. doi: 10.1016/j.jmb.2013.06.029. Epub 2013 Jun 28.
4
In vitro resistance mechanisms of Neisseria meningitidis against neutrophil extracellular traps.
Mol Microbiol. 2013 Aug;89(3):433-49. doi: 10.1111/mmi.12288. Epub 2013 Jun 25.
5
Phosphoethanolamine residues on the lipid A moiety of Neisseria gonorrhoeae lipooligosaccharide modulate binding of complement inhibitors and resistance to complement killing.
Infect Immun. 2013 Jan;81(1):33-42. doi: 10.1128/IAI.00751-12. Epub 2012 Oct 15.
6
Lack of lipid A pyrophosphorylation and functional lptA reduces inflammation by Neisseria commensals.
Infect Immun. 2012 Nov;80(11):4014-26. doi: 10.1128/IAI.00506-12. Epub 2012 Sep 4.
7
A novel mechanism of high-level, broad-spectrum antibiotic resistance caused by a single base pair change in Neisseria gonorrhoeae.
mBio. 2011 Sep 20;2(5). doi: 10.1128/mBio.00187-11. Print 2011.
8
Phosphoethanolamine substitution of lipid A and resistance of Neisseria gonorrhoeae to cationic antimicrobial peptides and complement-mediated killing by normal human serum.
Infect Immun. 2009 Mar;77(3):1112-20. doi: 10.1128/IAI.01280-08. Epub 2008 Dec 29.
9
Cationic antimicrobial peptide resistance in Neisseria meningitidis.
J Bacteriol. 2005 Aug;187(15):5387-96. doi: 10.1128/JB.187.15.5387-5396.2005.
10
Comparative whole-genome analyses reveal over 100 putative phase-variable genes in the pathogenic Neisseria spp.
Microbiology (Reading). 2001 Aug;147(Pt 8):2321-2332. doi: 10.1099/00221287-147-8-2321.
Zotero 插件