Ghanekar S A, Cecilia D, Kutubuddin M, Goverdhan M K, Dandawate C N, Banerjee K, Ghosh S N
National Institute of Virology, Pune.
Indian J Med Res. 1991 May;93:131-9.
Anti-idiotypic antibodies (anti-Ids, Ab2s) were prepared by immunizing rabbits with two murine monoclonal antibodies (Ab1) having specificities for two independent haemagglutinin (HA) epitopes on JE virus [viz., Hs-1, monoclonal antibody (MAb) specific for Japanese encephalitis virus (JEV) and Hx-1, MAb common to flaviviruses]. Anti-Hs-1 (S-Ab2) and Anti-Hx-1 (X-Ab2) reacted specifically with the immunizing Ab1. In addition, they could react with other MAbs whose reactivity was similar to their immunizing homologous Ab1. The paratope inhibition assay indicated that both anti-idiotypes recognized paratope related idiotopes on their respective Ab1 and could therefore be designated as Ab2 beta. Experimental animals (Swiss mice, Balb/c mice and guineapigs) immunized with S-Ab2 or X-Ab2 produced anti-JE virus antibodies (Ab3) which could be detected by enzyme linked immunosorbent assay, immunofluorescence, haemagglutination inhibition and neutralization tests. The anti-idiotypes were also found to stimulate a cellular immune response in vitro as assessed by 3H thymidine incorporation by lymphocytes from JE vaccinated individuals and experimentally immunized Balb/c mice. The findings of the present study suggest that both the anti-Id antibodies are homobodies which may act as surrogate antigens to manipulate the immune response against JEV.
通过用两种针对日本脑炎病毒（JE病毒）上两个独立血凝素（HA）表位具有特异性的鼠单克隆抗体（Ab1）免疫兔子，制备了抗独特型抗体（抗Id，Ab2）[即，Hs-1，针对日本脑炎病毒（JEV）的单克隆抗体（MAb）和Hx-1，黄病毒属共有的MAb]。抗Hs-1（S-Ab2）和抗Hx-1（X-Ab2）与免疫用Ab1发生特异性反应。此外，它们还能与其他反应性与其免疫同源Ab1相似的MAb发生反应。抗原结合位点抑制试验表明，两种抗独特型抗体都识别各自Ab1上与抗原结合位点相关的独特型表位，因此可被指定为Ab2β。用S-Ab2或X-Ab2免疫的实验动物（瑞士小鼠、Balb/c小鼠和豚鼠）产生了抗JE病毒抗体（Ab3），这些抗体可通过酶联免疫吸附测定、免疫荧光、血凝抑制和中和试验检测到。通过用来自接种JE疫苗的个体和实验性免疫的Balb/c小鼠的淋巴细胞掺入3H胸腺嘧啶核苷来评估，还发现抗独特型抗体在体外刺激细胞免疫反应。本研究结果表明，两种抗Id抗体都是同型抗体，它们可能作为替代抗原，来调控针对JEV的免疫反应。
