Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture , 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
J Agric Food Chem. 2014 Jun 4;62(22):5156-62. doi: 10.1021/jf5008117. Epub 2014 May 27.
A protein transiently expressed in the neural precursors of developing tissues (TENP) was found to be present in emu (Dromaius novaehollandiae) egg white as one of the major proteins. Nucleotide analysis of its encoding cDNA revealed a sequence of 452 amino acids including a 19 amino acid peptide signal. Phylogenetic analysis determined that emu TENP was clustered within the bactericidal/permeability-increasing protein (BPI) superfamily together with other avian TENPs. RT-PCR analysis revealed that the emu TENP gene was highly expressed in the magnum of the oviduct, indicating that TENP is a major egg white component. Emu TENP was purified by anion exchange chromatography and ammonium sulfate fractionation. Unlike BPI, emu TENP exhibited antibacterial activity against Gram-positive bacteria, including Micrococcus luteus and Bacillus subtilis, but not against Gram-negative bacteria such as Escherichia coli and Salmonella Typhimurium. The results suggest that emu TENP is a potent novel antibacterial protein with a spectrum distinct from that of BPI.
一种在发育组织的神经前体细胞中瞬时表达的蛋白质（TENP）被发现存在于鸸鹋（Dromaius novaehollandiae）蛋清中，是主要蛋白质之一。对其编码 cDNA 的核苷酸分析显示，它包含一个 452 个氨基酸的序列，其中包括一个 19 个氨基酸的肽信号。系统发育分析确定，鸸鹋 TENP 与其他禽类 TENP 一起聚类在杀菌/通透性增加蛋白（BPI）超家族中。RT-PCR 分析显示，鸸鹋 TENP 在输卵管的膨大部高度表达，表明 TENP 是蛋清的主要成分之一。通过阴离子交换层析和硫酸铵分级分离纯化了鸸鹋 TENP。与 BPI 不同，鸸鹋 TENP 对革兰氏阳性菌（包括藤黄微球菌和枯草芽孢杆菌）具有抗菌活性，但对革兰氏阴性菌（如大肠杆菌和鼠伤寒沙门氏菌）没有活性。结果表明，鸸鹋 TENP 是一种具有独特抗菌谱的新型强效抗菌蛋白。
