Leite Daniel Silva, Sandrini-Neto Leonardo, Camargo Manuela Zeglin, Thomas Micheli Cristina, Lana Paulo C
Centro de Estudos do Mar, Universidade Federal do Paraná, Av. Beira Mar s/n, PO Box 61, CEP 83255-976 Pontal do Paraná, Paraná, Brazil.
Universidade do Estado de Santa Catarina, Av. Madre Benvenuta 2007, CEP 88035-001 Florianópolis, Santa Catarina, Brazil.
Mar Pollut Bull. 2014 Jun 15;83(1):38-47. doi: 10.1016/j.marpolbul.2014.04.035. Epub 2014 May 10.
This study assesses through a multiple before-after-control-impact (MBACI) design the effects of diesel oil on the structure of nematode assemblages in unvegetated tidal flats of a subtropical estuary. Oil-exposed treatments were contrasted with controls for a duration of four successive days before and after an experimental spill in three distinct areas of the Paranaguá Estuarine Complex (Southern Brazil). No significant differences were observed in nematode total density, number of taxa and the overall assemblage structure between the control and impact treatments from before to after the experimental spill. This reinforces the idea that, despite being good indicators of environmental stress, free-living marine nematodes are able to tolerate low concentrations of hydrocarbons and to survive in moderately contaminated areas. We also show that robust experimental designs are useful to avoid confounding expected natural variability with the effects of a mild impact.
本研究通过多组前后对照影响（MBACI）设计，评估了柴油对亚热带河口无植被潮滩中线虫群落结构的影响。在巴拉那瓜河口复合体（巴西南部）的三个不同区域进行实验性溢油前后，连续四天将受油暴露处理与对照进行对比。在实验性溢油前后，对照处理和受影响处理之间的线虫总密度、分类单元数量和整体群落结构均未观察到显著差异。这强化了这样一种观点，即尽管自由生活的海洋线虫是环境压力的良好指标，但它们能够耐受低浓度的碳氢化合物，并在中度污染区域生存。我们还表明，稳健的实验设计有助于避免将预期的自然变异性与轻度影响的效应混淆。
