Marques Joseane A, de Assis Helena C Silva, Guiloski Izonete C, Sandrini-Neto Leonardo, Carreira Renato S, Lana Paulo C
Laboratório de Bentos, Centro de Estudos do Mar, Universidade Federal do Paraná, Av. Beira Mar, s/n, CEP 83255-000 Pontal do Paraná, PR, Brazil.
Departamento de Farmacologia, Universidade Federal do Paraná, Caixa Postal 19031, CEP 81531-970 Curitiba, PR, Brazil.
Ecotoxicol Environ Saf. 2014 Sep;107:269-75. doi: 10.1016/j.ecoenv.2014.06.001. Epub 2014 Jul 8.
We evaluated the effects of diesel oil on the bivalve Mytella guyanensis using biomarkers of oxidative stress (glutathione S-transferase, glutathione peroxidase, and reduced glutathione) after an experimental in situ spill in a mangrove area in southern Brazil. A linear model was developed for the Multiple Before-After Control-Impact (MBACI) experimental design to assess the significance of biological responses. Control and impacted sites were sampled seven and two days before as well as two and seven days after the spill. With the exception of a late response of reduced glutathione (GSH) levels on day seven, none of the biomarkers were significantly altered by the impact. This result was attributed to the high environmental variability of the experimental sites combined with a low sensitivity of Mytella guyanensis to diesel oil at short time-scales. The high resistance of M. guyanensis suggests that its antioxidant response is triggered only after a medium- to long-term exposure to contaminants.
在巴西南部一个红树林地区进行原位溢油实验后，我们使用氧化应激生物标志物（谷胱甘肽S-转移酶、谷胱甘肽过氧化物酶和还原型谷胱甘肽）评估了柴油对双壳贝类圭亚那贻贝的影响。针对多组前后对照-影响（MBACI）实验设计建立了线性模型，以评估生物反应的显著性。在溢油前7天和2天以及溢油后2天和7天对对照点和受影响点进行了采样。除了第7天还原型谷胱甘肽（GSH）水平出现延迟反应外，没有任何生物标志物因该影响而发生显著变化。这一结果归因于实验地点的高环境变异性以及圭亚那贻贝在短时间尺度上对柴油的低敏感性。圭亚那贻贝的高抗性表明，其抗氧化反应仅在中长期接触污染物后才会被触发。
