Franck J, Fried G, Brodin E
Department of Physiology, Karolinska Institutet, Stockholm, Sweden.
Eur J Pharmacol. 1989 Dec 12;174(1):85-90. doi: 10.1016/0014-2999(89)90877-7.
The effect of the tachykinin neuropeptides, substance P (SP), neurokinin A (NKA) and the neurokinin B (NKB) receptor agonist, senktide, on the potassium-evoked release of endogenous serotonin (5-hydroxytryptamine, 5-HT) was investigated in superfused tissue slices of rat ventral spinal cord, where 5-HT is known to coexist with SP. Endogenous 5-HT was assayed by HPLC with electrochemical detection. The evoked release of 5-HT was significantly enhanced by 10(-4) M SP (190% increase) and 10(-5) M SP (74% increase) but not by 10(-6) M SP, NKA (10(-5) and 10(-4) M) and senktide (10(-5) and 10(-4) M) had no significant effect on the 5-HT release. The results suggest that, in the rat ventral spinal cord, where most of the 5-HT and SP is stored in the same nerve endings. SP but not NKA nor NKB potentiates the evoked release of 5-HT in a dose-dependent manner.
在大鼠腹侧脊髓的灌流组织切片中，研究了速激肽神经肽P物质（SP）、神经激肽A（NKA）以及神经激肽B（NKB）受体激动剂senktide对钾离子诱发的内源性5-羟色胺（5-HT）释放的影响，已知在该组织中5-HT与SP共存。采用高效液相色谱电化学检测法测定内源性5-HT。10(-4) M的SP（增加190%）和10(-5) M的SP（增加74%）可显著增强5-HT的诱发释放，但10(-6) M的SP无此作用，NKA（10(-5) 和10(-4) M）以及senktide（10(-5) 和10(-4) M）对5-HT释放无显著影响。结果表明，在大鼠腹侧脊髓中，大部分5-HT和SP储存于相同的神经末梢，SP而非NKA或NKB以剂量依赖方式增强5-HT的诱发释放。
