Gellersen B, DiMattia G E, Friesen H G, Bohnet H G
Institute for Hormone and Fertility Research, Hamburg, F.R.G.
Mol Cell Endocrinol. 1989 Oct;66(2):153-61. doi: 10.1016/0303-7207(89)90027-0.
The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) stimulated prolactin (PRL) release from the PRL producing human B-lymphoblastoid cell line IM-9-P3 within 30 min with an EC50 of 5 x 10(-9) M. Increased release was entirely attributable to a loss from intracellular PRL pools. No change in hPRL mRNA was observed during 8 h of exposure to TPA. Prolonged exposure of the cells to 2 x 10(-7) M TPA, however, led to a maximal reduction of hPRL mRNA levels after 24 h and a subsequent recovery by 72 h. Secretory rates followed a corresponding kinetic. The relative abundance of c-myc mRNA was not affected, although a persistent inhibition of cellular proliferation occurred upon chronic exposure to TPA. The addition of dibutyryl cAMP caused a minor transient increase in hPRL secretion by 35% after 1 h.
佛波酯12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）在30分钟内刺激了泌乳素（PRL）从产生PRL的人B淋巴细胞系IM - 9 - P3中释放，其半数有效浓度（EC50）为5×10⁻⁹ M。释放增加完全归因于细胞内PRL池的减少。在暴露于TPA的8小时内，未观察到hPRL mRNA有变化。然而，将细胞长时间暴露于2×10⁻⁷ M TPA后，24小时后hPRL mRNA水平最大程度降低，随后在72小时恢复。分泌率呈现相应的动力学变化。尽管长期暴露于TPA会持续抑制细胞增殖，但c - myc mRNA的相对丰度未受影响。加入二丁酰cAMP后，1小时后hPRL分泌轻微短暂增加35%。
