Wheeler A W, Johansson S G
Institutionen för Klinisk Immunologi, Karolinska Sjukhuset, Stockholm, Sweden.
Int Arch Allergy Appl Immunol. 1989;90(4):320-5. doi: 10.1159/000235047.
An in vivo system is described in which penicilloyl antibody was produced from peripheral leucocytes of a grass pollen-sensitive patient who had received penicillin therapy, by challenge of the cells with penicilloyl-grass pollen extract conjugate. Incubation of these leucocytes with a number of modified preparations of grass pollen extract with various T-cell-stimulating properties was shown to affect penicilloyl antibody production. Both chymotryptically fragmented rye grass pollen extract and a conjugate of f met-leu-phe and rye grass pollen extract enhanced penicilloyl-specific antibody similarly to the enhancement induced by unmodified extract, though at high concentration some suppression was seen. A conjugate of polysarcosine and rye grass pollen extract, previously shown to cause antibody suppression in mice, was similarly suppressive for penicilloyl-specific antibody. The system therefore shows potential for the evaluation of the effects of modified allergen treatment on antibody levels via T-cell mechanisms.
本文描述了一种体内系统，在该系统中，通过用青霉噻唑-草花粉提取物偶联物刺激细胞，从接受过青霉素治疗的草花粉敏感患者的外周血白细胞中产生青霉噻唑抗体。结果显示，将这些白细胞与多种具有不同T细胞刺激特性的改良草花粉提取物制剂共同孵育，会影响青霉噻唑抗体的产生。胰凝乳蛋白酶消化片段化的黑麦草花粉提取物以及f蛋氨酸-亮氨酸-苯丙氨酸与黑麦草花粉提取物的偶联物，与未修饰提取物诱导的增强作用相似，均能增强青霉噻唑特异性抗体，不过在高浓度时会出现一些抑制作用。先前已证明聚肌氨酸与黑麦草花粉提取物的偶联物会在小鼠中导致抗体抑制，对青霉噻唑特异性抗体同样具有抑制作用。因此，该系统显示出通过T细胞机制评估改良变应原治疗对抗体水平影响的潜力。
