Standring R, Lavender E A, Wheeler A W, Spackman V M, Moran D M
Bencard Allergy Unit, Beecham Pharmaceuticals, Epsom, Surrey, England.
Int Arch Allergy Appl Immunol. 1988;87(4):337-41. doi: 10.1159/000234698.
Rye grass pollen extract was digested by chymotrypsin to produce fragments with a molecular weight below 10,000, as demonstrated by polyacrylamide gel electrophoresis. Chymotryptic fragments did not react with either human or mouse IgG antibodies specific for rye grass pollen, nor did they induce an antibody response in mice with specificity for the parent extract. However, macrophage-presented fragments retained the ability to react with rye-specific T cells in a lymphoproliferation assay. Furthermore, these fragments induced the development of splenocytes capable of supporting dinitrophenyl specific antibody production. This implies that the fragments still react with, and induce, rye grass pollen extract-specific T-helper cells. The possibility that such fragments might have potential for use in immunotherapy for the specific treatment of allergy is discussed.
经聚丙烯酰胺凝胶电泳证实，黑麦草花粉提取物经胰凝乳蛋白酶消化后产生分子量低于10000的片段。胰凝乳蛋白酶消化后的片段既不与针对黑麦草花粉的人或小鼠IgG抗体发生反应，也不会在小鼠体内诱导产生针对亲本提取物的抗体反应。然而，巨噬细胞呈递的片段在淋巴细胞增殖试验中仍保留与黑麦特异性T细胞发生反应的能力。此外，这些片段诱导了能够支持二硝基苯基特异性抗体产生的脾细胞的发育。这意味着这些片段仍然与黑麦草花粉提取物特异性T辅助细胞发生反应并诱导其产生。文中讨论了此类片段可能具有用于过敏特异性治疗的免疫疗法的潜力。
