Nassif Nicholas D, Cambray Samantha E, Kraut Daniel A
Department of Chemistry, Villanova University, Villanova, PA, USA.
IUBMB Life. 2014 May;66(5):309-17. doi: 10.1002/iub.1271. Epub 2014 May 14.
ATP-dependent proteases are present in all organisms, where they are responsible for much of intracellular protein degradation. Most proteins are processively unfolded and degraded into small peptides; however, in a few so-called slippery substrates, the protease stalls at a folded domain and releases a large protein fragment. In this review, we describe the properties of physiological slippery substrates that are processed in this manner by ATP-dependent proteases and the recent advances that have been made in understanding the mechanism underlying their partial degradation.
ATP 依赖性蛋白酶存在于所有生物体中，它们负责细胞内大部分蛋白质的降解。大多数蛋白质会被逐步展开并降解为小肽；然而，在少数所谓的易滑底物中，蛋白酶会在一个折叠结构域处停滞，并释放出一个大的蛋白质片段。在这篇综述中，我们描述了以这种方式被 ATP 依赖性蛋白酶加工的生理性易滑底物的特性，以及在理解其部分降解背后机制方面取得的最新进展。
