Prajapati Prajesh, Vaghela Vipul, Rawtani Deepak, Patel Harshad, Kubavat Jasmin, Baraiya Dharmendra
Department of Pharmaceutical Analysis, Institute of Research and Development, Gujarat Forensic Sciences University, Sector-18A, Nr. Police bhavan, Gandhinagar 382007, Gujarat, India.
A.R. College of Pharmacy and G.H. Patel Institute of Pharmacy, Vallabh Vidyanagar 388120, Gujarat, India.
J Pharm Anal. 2012 Aug;2(4):306-309. doi: 10.1016/j.jpha.2012.02.004. Epub 2012 Feb 28.
A novel, safe, economic and sensitive method of spectrophotometric estimation has been developed using Azeoptropic mixture (water:methanol: 60:40, v/v) for the quantitative determination of Lornoxicam, a practically water-insoluble drug. Hence, Lornoxicam stock solution was prepared in Azeoptropic mixture. Lornoxicam showed maximum absorbance at 383 nm. Beer's law was obeyed in the concentration range 4-24 μg/mL with regression coefficient of 0.999. The method was validated in terms of linearity (=0.999), precision (CV for intra-day and inter-day was 0.28-0.68 and 0.12-0.92, respectively), accuracy (98.03-100.59% w/w) and specificity. This method is simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.
已开发出一种新颖、安全、经济且灵敏的分光光度法估算方法，该方法使用共沸混合物（水：甲醇：60:40，v/v）对洛索洛芬（一种几乎不溶于水的药物）进行定量测定。因此，在共沸混合物中制备了洛索洛芬储备溶液。洛索洛芬在383 nm处显示出最大吸光度。在4 - 24 μg/mL的浓度范围内符合比尔定律，回归系数为0.999。该方法在线性（=0.999）、精密度（日内和日间的CV分别为0.28 - 0.68和0.12 - 0.92）、准确度（98.03 - 100.59% w/w）和专属性方面得到了验证。该方法简单、精密、准确、灵敏且可重现，可用于市售制剂的常规质量控制检测。
