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一点红正己烷提取物的体外抗氧化活性及高效薄层色谱法测定

In vitro antioxidant activity and HPTLC determination of n-hexane extract of Emilia sonchifolia (L.)DC.

作者信息

Sophia D, Ragavendran P, Arulraj C, Gopalakrishnan V K

机构信息

Department of Biochemistry, Karpagam University, Coimbatore - 641 021 (T.N.), India.

出版信息

J Basic Clin Pharm. 2011 Sep;2(4):179-83. Epub 2011 Nov 15.

Abstract

The free radical scavenging activities of n-hexane extract of the whole plant of Emilia sonchifolia was evaluated by employing various in vitro assay systems like DPPH radical scavenging activity, superoxide radical scavenging activity and hydrogen peroxide scavenging activity with IC50 values 180, 160 and 160 μg/ml respectively. The results of the study indicate that the n-hexane extract of the whole plant of Emilia sonchifolia possess a significant scavenging effect with increasing concentrations probably due to its antioxidant potential. High performance thin layer chromatography (HPTLC) analysis in the n-hexane extract of Emilia sonchifolia showed the presence of terpenoids which probably may be responsible for the antioxidant activity. Thus, n-hexane extract of Emilia sonchifolia can be used potentially as a bioactive source of natural antioxidants due to the presence of terpenoids in it.

摘要

通过采用多种体外测定系统,如DPPH自由基清除活性、超氧阴离子自由基清除活性和过氧化氢清除活性,对一点红全草正己烷提取物的自由基清除活性进行了评估,其IC50值分别为180、160和160μg/ml。研究结果表明,一点红全草正己烷提取物随着浓度增加具有显著的清除作用,这可能归因于其抗氧化潜力。对一点红正己烷提取物进行的高效薄层色谱(HPTLC)分析表明存在萜类化合物,这可能是其抗氧化活性的原因。因此,由于一点红正己烷提取物中存在萜类化合物,它有可能作为天然抗氧化剂的生物活性来源。

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