凤尾草（Linn.）DC 对雄性白化大鼠胰腺酒精诱导氧化应激的影响。

Effect of Emilia sonchifolia (Linn.)DC on alcohol-induced oxidative stress in pancreas of male albino rats.

机构信息

Department of Biochemistry, Karpagam University, Coimbatore, T.N., India.

出版信息

Asian Pac J Trop Med. 2011 Dec;4(12):973-7. doi: 10.1016/S1995-7645(11)60229-0.

DOI:10.1016/S1995-7645(11)60229-0

PMID:22118034

Abstract

OBJECTIVE

To explore the efficacy of n-hexane extract of Emilia sonchifolia (E. sonchifobia) against ethanol induced pancreatic dysfunction in the young Wistar albino rats.

METHODS

The rats were divided into four groups. Control rats in group received distilled water orally, group received oral administration of 20% (w/v) ethanol dissolved in drinking water, group received oral administration of 20% (w/v) ethanol in distilled water+n-hexane extract of E. sonchifolia (250 mg/kg body weight), and group received oral administration of n-hexane extract of E. sonchifolia (250 mg/kg body weight) alone. Liver marker enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), pancreatic enzymatic antioxidants superoxide dismutase, lipid peroxidation, catalase, glutathione peroxidase, non-enzymatic antioxidants glutathione and vitamin C were measured and compared.

RESULTS

Administration of 20% ethanol for 16 weeks significantly increased the liver marker enzymes AST, ALT(P<0.05), reduced the pancreatic enzymatic antioxidants superoxide dismutase, lipid peroxidation, catalase, glutathione peroxidase, glutathione and vitamin C(P<0.05). Histopathological examination showed that the ethanol provoked the oxidative stress which was demonstrated as pancreatic necrosis and oedema. Simultaneous administration of n-hexane extract of E. sonchifolia (250 mg/kg body weight) protected the pancreas against the damage induced by ethanol which was confirmed by the histopathological studies and the normalization of biochemical parameters.

CONCLUSIONS

Thus n-hexane extract of E. sonchifolia shows a promise in therapeutic use in alcohol induced oxidative stress.

摘要

目的

探索艾草正己烷提取物（E. sonchifobia）对未成年 Wistar 白化大鼠乙醇诱导的胰腺功能障碍的疗效。

方法

将大鼠分为四组。对照组大鼠口服蒸馏水，组给予 20%（w/v）乙醇溶解于饮用水中，组给予 20%（w/v）乙醇+艾草正己烷提取物（250mg/kg 体重），组给予艾草正己烷提取物（250mg/kg 体重）。测量并比较各组大鼠的肝标志物酶天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、胰腺酶抗氧化剂超氧化物歧化酶、脂质过氧化、过氧化氢酶、谷胱甘肽过氧化物酶、非酶抗氧化剂谷胱甘肽和维生素 C。

结果

16 周给予 20%乙醇可显著增加肝标志物酶 AST、ALT（P<0.05），降低胰腺酶抗氧化剂超氧化物歧化酶、脂质过氧化、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽和维生素 C（P<0.05）。组织病理学检查显示，乙醇引起了氧化应激，表现为胰腺坏死和水肿。同时给予艾草正己烷提取物（250mg/kg 体重）可保护胰腺免受乙醇诱导的损伤，这一点可通过组织病理学研究和生化参数的正常化得到证实。