ATP 水解对耳蜗内毛细胞中 CaV1.3 通道的功能至关重要，通过为 Ca2+ 清除提供燃料。
ATP hydrolysis is critically required for function of CaV1.3 channels in cochlear inner hair cells via fueling Ca2+ clearance.
机构信息
InnerEarLab, Department of Otolaryngology, University Medical Center Göttingen, 37099 Göttingen, Germany, International Max-Planck Research School Neuroscience, Göttingen Graduate School for Neuroscience and Molecular Biosciences, and.
InnerEarLab, Department of Otolaryngology, University Medical Center Göttingen, 37099 Göttingen, Germany, International Max-Planck Research School Molecular Biology, Göttingen Graduate School for Neuroscience and Molecular Biosciences, University of Göttingen, 37077 Göttingen, Germany, and.
出版信息
J Neurosci. 2014 May 14;34(20):6843-8. doi: 10.1523/JNEUROSCI.4990-13.2014.
Abstract
Sound encoding is mediated by Ca(2+) influx-evoked release of glutamate at the ribbon synapse of inner hair cells. Here we studied the role of ATP in this process focusing on Ca(2+) current through CaV1.3 channels and Ca(2+) homeostasis in mouse inner hair cells. Patch-clamp recordings and Ca(2+) imaging demonstrate that hydrolyzable ATP is essential to maintain synaptic Ca(2+) influx in inner hair cells via fueling Ca(2+)-ATPases to avoid an increase in cytosolic [Ca(2+)] and subsequent Ca(2+)/calmodulin-dependent inactivation of CaV1.3 channels.
摘要
声音编码是由内毛细胞的带状突触中 Ca(2+)内流引发的谷氨酸释放介导的。在这里，我们研究了 ATP 在这个过程中的作用，重点是 CaV1.3 通道中的 Ca(2+)电流和 Ca(2+)在内耳毛细胞中的稳态。膜片钳记录和 Ca(2+)成像表明，可水解的 ATP 对于通过为 Ca(2+)-ATP 酶提供燃料来维持内毛细胞中的突触 Ca(2+)内流是必不可少的，以避免细胞浆 [Ca(2+)]增加和随后的 Ca(2+)/钙调蛋白依赖性 CaV1.3 通道失活。
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