Tamamoto Takashi, Ohno Koichi, Goto-Koshino Yuko, Tsujimoto Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
J Vet Med Sci. 2014 Aug;76(8):1183-8. doi: 10.1292/jvms.14-0108. Epub 2014 May 15.
The serum amyloid A (SAA) concentration is higher in mammary tumors with metastases in both humans and animals. In the present study, the direct effects of recombinant feline SAA (rfSAA) protein on invasiveness of feline mammary carcinoma cells were evaluated. As an indicator of invasiveness, matrix metalloproteinase-9 (MMP-9) expression was investigated in 4 feline mammary carcinoma cell lines of different origins. In 3 of 4 cell lines, MMP-9 expression was significantly increased by rfSAA stimulation. The invasive capacities of feline mammary carcinoma cells were also stimulated by rfSAA. The findings of this study have identified a novel role for SAA in mammary tumorigenesis and suggest that therapeutic strategies targeting SAA may provide new alternatives in treating tumor invasion and metastasis.
在人和动物中，发生转移的乳腺肿瘤中血清淀粉样蛋白A（SAA）浓度更高。在本研究中，评估了重组猫SAA（rfSAA）蛋白对猫乳腺癌细胞侵袭性的直接影响。作为侵袭性的指标，在4种不同来源的猫乳腺癌细胞系中研究了基质金属蛋白酶-9（MMP-9）的表达。在4种细胞系中的3种中，rfSAA刺激显著增加了MMP-9的表达。rfSAA也刺激了猫乳腺癌细胞的侵袭能力。本研究结果确定了SAA在乳腺肿瘤发生中的新作用，并表明针对SAA的治疗策略可能为治疗肿瘤侵袭和转移提供新的选择。
