Chen Weiwei, Sun Shangde, Liang Shaohua, Peng Le, Wang Yadong, Shen Mi
Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology.
J Oleo Sci. 2014;63(6):619-28. doi: 10.5650/jos.ess13189. Epub 2014 May 15.
Lipase-catalyzed hydrolysis of linseed oil was investigated. Four commercially available microbial lipases of Lipase AY, Lipozyme RMIM, Lipozyme TLIM, and Novozym 435 were used. Among these tested lipases, Lipase AY exhibited the best hydrolysis effeciency to linseed oil. The effect of reaction variables was also evaluated and optimized using response surface methodology. A second-order regression for the Box-Behken design was used to study the effect of five independent variables, such as, temperature, pH, oil-aqueous phase ratio, enzyme load, and reaction time, on the hydrolysis of linseed oil. The optimal conditions were as follows: temperature 33°C, pH 5.80, oil-aqueous phase ratio 0.90 (w/w), enzyme load 1.20% (relative to the weight of total substrates), and reaction time 3.33 h. Under these conditions, the hydrolysis ratio of linseed oil was 93.92±0.54%.
研究了脂肪酶催化的亚麻籽油水解反应。使用了四种市售的微生物脂肪酶，即脂肪酶AY、Lipozyme RMIM、Lipozyme TLIM和诺维信435。在这些测试的脂肪酶中，脂肪酶AY对亚麻籽油表现出最佳的水解效率。还使用响应面法评估并优化了反应变量。采用Box-Behken设计的二阶回归来研究五个自变量，即温度、pH值、油-水相比、酶负载量和反应时间，对亚麻籽油水解的影响。最佳条件如下：温度33°C，pH值5.80，油-水相比0.90（w/w），酶负载量1.20%（相对于总底物重量），反应时间3.33小时。在这些条件下，亚麻籽油的水解率为93.92±0.54%。
