Laboratoire de Biochimie et Biologie Moléculaire Végétales, Campus Grimaldi, Université de Corse, CNRS UMR6134 SPE, BP52, 20250 Corte, France.
Int J Mol Sci. 2023 Jul 31;24(15):12274. doi: 10.3390/ijms241512274.
Green leaf volatiles (GLVs), including short chain volatile aldehydes, are widely used in the flavor and food industries because of their fresh aroma. To meet the growing demand for natural GLVs with high added value, the use of biocatalytic processes appears as a relevant application. In such processes, vegetable oils are bioconverted into GLVs. First, the triacylglycerols of the oils are hydrolyzed by a lipase. Then, the free polyunsaturated fatty acids are converted by a lipoxygenase. Finally, volatile C6 or C9 aldehydes and 9- or 12-oxoacids are produced with a hydroperoxide lyase. Optimization of each biocatalytic step must be achieved to consider a scale-up. In this study, three oils (sunflower, hempseed, and linseed oils) and three lipases (, , and lipases) have been tested to optimize the first step of the process. The experimental design and response surface methodology (RSM) were used to determine the optimal hydrolysis conditions for each oil. Five factors were considered, i.e., pH, temperature, reaction duration, enzyme load, and oil/aqueous ratio of the reaction mixture. lipase was selected as the most efficient enzyme to achieve conversion of 96 ± 1.7%, 97.2 ± 3.8%, and 91.8 ± 3.2%, respectively, for sunflower, hempseed, and linseed oils under the defined optimized reaction conditions.
绿叶挥发物（GLVs），包括短链挥发性醛类，因其新鲜的香气而广泛应用于香精和食品工业。为了满足对具有高附加值的天然 GLVs 的日益增长的需求，生物催化工艺的应用似乎具有重要意义。在这些过程中，植物油被生物转化为 GLVs。首先，脂肪酶将油中的三酰基甘油水解。然后，脂氧合酶将游离的多不饱和脂肪酸转化。最后，用过氧化物裂解酶生成挥发性 C6 或 C9 醛和 9-或 12-氧代酸。必须优化每个生物催化步骤以考虑扩大规模。在这项研究中，测试了三种油（葵花籽油、大麻籽油和亚麻籽油）和三种脂肪酶（、和脂肪酶），以优化该过程的第一步。实验设计和响应面法（RSM）用于确定每种油的最佳水解条件。考虑了五个因素，即 pH 值、温度、反应时间、酶负荷和反应混合物的油/水比例。选择脂肪酶作为最有效的酶，在定义的最佳反应条件下，分别实现了葵花籽油、大麻籽油和亚麻籽油转化率为 96±1.7%、97.2±3.8%和 91.8±3.2%。
