Alout Haoues, Yameogo Bienvenue, Djogbénou Luc Salako, Chandre Fabrice, Dabiré Roch Kounbobr, Corbel Vincent, Cohuet Anna
Institut de Recherche pour le Développement, Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle, UM1-UM2-CNRS5290-IRD 224, Montpellier, France Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso.
Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso.
J Infect Dis. 2014 Nov 1;210(9):1464-70. doi: 10.1093/infdis/jiu276. Epub 2014 May 14.
Despite its epidemiological importance, the impact of insecticide resistance on vector-parasite interactions and malaria transmission is poorly understood. Here, we explored the impact of Plasmodium infection on the level of insecticide resistance to dichlorodiphenyltrichloroethane (DDT) in field-caught Anopheles gambiae sensu stricto homozygous for the kdr mutation. Results showed that kdr homozygous mosquitoes that fed on infectious blood were more susceptible to DDT than mosquitoes that fed on noninfectious blood during both ookinete development (day 1 after the blood meal) and oocyst maturation (day 7 after the blood meal) but not during sporozoite invasion of the salivary glands. Plasmodium falciparum infection seemed to impose a fitness cost on mosquitoes by reducing the ability of kdr homozygous A. gambiae sensu stricto to survive exposure to DDT. These results suggest an interaction between Plasmodium infection and the insecticide susceptibility of mosquitoes carrying insecticide-resistant alleles. We discuss this finding in relation to vector control efficacy.
尽管杀虫剂抗性具有流行病学重要性，但其对媒介-寄生虫相互作用和疟疾传播的影响仍知之甚少。在此，我们探究了疟原虫感染对野外捕获的、纯合携带击倒抗性（kdr）突变的冈比亚按蚊（Anopheles gambiae sensu stricto）对滴滴涕（DDT）的杀虫剂抗性水平的影响。结果显示，在动合子发育阶段（血餐进食后第1天）和卵囊成熟阶段（血餐进食后第7天），取食感染性血液的kdr纯合蚊子比取食非感染性血液的蚊子对DDT更敏感，但在子孢子侵入唾液腺阶段并非如此。恶性疟原虫感染似乎通过降低kdr纯合冈比亚按蚊对DDT暴露的存活能力，对蚊子施加了一种适合度代价。这些结果表明疟原虫感染与携带杀虫剂抗性等位基因的蚊子的杀虫剂易感性之间存在相互作用。我们结合病媒控制效果讨论了这一发现。
