预防龋齿的无药物疗法的发展。
The development of drug-free therapy for prevention of dental caries.
作者信息
Chen Fu, Jia Zhenshan, Rice Kelly C, Reinhardt Richard A, Bayles Kenneth W, Wang Dong
机构信息
Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE, 68198-6025, USA.
出版信息
Pharm Res. 2014 Nov;31(11):3031-7. doi: 10.1007/s11095-014-1396-1. Epub 2014 May 15.
PURPOSE
The purpose of this study was to develop a novel, drug-free therapy that can reduce the over-accumulation of cariogenic bacteria on dental surfaces.
METHODS
We designed and synthesized a polyethylene glycol (PEG)-based hydrophilic copolymer functionalized with a pyrophosphate (PPi) tooth-binding anchor using "click" chemistry. The polymer was then evaluated for hydroxyapatite (HA) binding kinetics and capability of reducing bacteria adhesion to artificial tooth surface.
RESULTS
The PPi-PEG copolymer can effectively inhibit salivary protein adsorption after rapid binding to an artificial tooth surface. As a result, the in vitro S. mutans adhesion study showed that the PPi-PEG copolymer can inhibit saliva protein-promoted S. mutans adhesion through the creation of a neutral, hydrophilic layer on the artificial tooth surface.
CONCLUSIONS
The results suggested the potential application of a PPi-PEG copolymer as a drug-free alternative to current antimicrobial therapy for caries prevention.
目的
本研究的目的是开发一种新型的无药物疗法，该疗法可减少牙面上致龋菌的过度积聚。
方法
我们使用“点击”化学设计并合成了一种基于聚乙二醇（PEG）的亲水性共聚物，该共聚物用焦磷酸盐（PPi）牙齿结合锚进行了功能化修饰。然后评估该聚合物与羟基磷灰石（HA）的结合动力学以及减少细菌粘附到人工牙表面的能力。
结果
PPi-PEG共聚物在快速结合到人工牙表面后可有效抑制唾液蛋白吸附。因此，体外变形链球菌粘附研究表明，PPi-PEG共聚物可通过在人工牙表面形成中性亲水性层来抑制唾液蛋白促进的变形链球菌粘附。
结论
结果表明PPi-PEG共聚物作为目前预防龋齿抗菌疗法的无药物替代方案具有潜在应用价值。
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