Bezerra José Artur Brilhante, da Silva Andréia Maria, Peixoto Gislayne Christianne Xavier, da Silva Mariana de Araújo, Franco de Oliveira Moacir, Silva Alexandre Rodrigues
Laboratory of Animal Germplasm Conservation - LCGA, Universidade Federal Rural do Semi-Árido - UFERSA, Mossoró, RN, Brazil.
Zoolog Sci. 2014 May;31(5):338-42. doi: 10.2108/zs130149.
In order to establish protocols for gamete recovery from accidentally killed wild animals, or to take advantage of those slaughtered by captive breeders, we assess the influence of two methods on the recovery of epididymal sperm from collared peccaries, and verify the effect of centrifugation on such gametes. Genitalia from nine animals were used. For each animal, one epididymis was processed by flotation and the other was processed by retrograde flushing, both using a buffered media based on Tris. Following recovery, sperm were evaluated for motility, vigor, viability, functional membrane integrity, and morphology. A 1-mL aliquot of each sample was centrifuged, the supernatant removed, and the pellet suspended and evaluated as fresh samples. The sperm characteristics did not differ between the samples collected by flotation or retrograde flushing (P < 0.05). Centrifugation promoted an increase in head and tail defects, thus reducing the percentage of viable sperm (P < 0.05). No other parameter assessed for both methods was affected by centrifugation. In conclusion, epididymal sperm from collared peccaries can be efficiently collected through flotation or retrograde flushing, but not when either is followed by centrifugation.
为了建立从意外死亡的野生动物中回收配子的方案，或利用圈养繁殖者宰杀的动物，我们评估了两种方法对领西貒附睾精子回收的影响，并验证了离心对这些配子的影响。使用了来自9只动物的生殖器。对于每只动物，一个附睾采用基于Tris的缓冲培养基通过漂浮法处理，另一个附睾采用逆行冲洗法处理。回收后，对精子的活力、活力、生存力、功能膜完整性和形态进行评估。将每个样品的1 mL等分试样离心，去除上清液，将沉淀悬浮并作为新鲜样品进行评估。通过漂浮或逆行冲洗收集的样品之间精子特征没有差异（P < 0.05）。离心导致头部和尾部缺陷增加，从而降低了活精子的百分比（P < 0.05）。两种方法评估的其他参数均不受离心影响。总之，领西貒的附睾精子可以通过漂浮或逆行冲洗有效收集，但在任何一种方法后进行离心则不行。
