Phurimsak Chayakom, Yildirim Ender, Tarn Mark D, Trietsch Sebastiaan J, Hankemeier Thomas, Pamme Nicole, Vulto Paul
Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Lab Chip. 2014 Jul 7;14(13):2334-43. doi: 10.1039/c4lc00139g. Epub 2014 May 16.
We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air-liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin-biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.
我们开发了一种基于磁性颗粒的检测平台，其中功能化磁性颗粒依次通过层叠的试剂和洗涤缓冲液体积。水性液体的层叠通过相引导技术实现；这种微结构在溶液进入微流控腔室时控制其前进的气液界面。这允许手动填充设备，无需外部泵送系统，并且系统的准备仅需几分钟。在这里，我们将该平台应用于两种芯片上的策略：（i）一步法链霉亲和素-生物素结合检测，以及（ii）两步法C反应蛋白免疫检测。通过这些，我们展示了将多个反应和洗涤过程浓缩为一步如何显著减少操作时间，两种检测程序都只需不到8秒。
