Csiba A, Szécsényi-Nagy L
Acta Vet Hung. 1989;37(3):191-6.
A new method is reported for staining and evaluating gel electropherograms of proteins. With pyrogallol red-molybdate reagent the gel-embedded proteins are transformed into a derivative of blue colour. After destaining, the blue-coloured proteins are well visible against a colourless background and can be quantified by densitometry with high reliability. The quantity of the coloured protein is directly proportional to the height of peaks in the densitogram. Colour intensity is concentration dependent. The measurement range of serum albumin was 1 to 50 micrograms/tube and 10 to 100 micrograms/slab in polyacrylamide gel disc electrophoresis and agar gel electrophoresis, respectively.
报道了一种用于蛋白质凝胶电泳图谱染色和评估的新方法。使用连苯三酚红-钼酸盐试剂，将凝胶包埋的蛋白质转化为蓝色衍生物。脱色后，蓝色蛋白质在无色背景下清晰可见，可通过光密度测定法进行高度可靠的定量。有色蛋白质的量与密度图中峰的高度成正比。颜色强度取决于浓度。在聚丙烯酰胺凝胶圆盘电泳和琼脂凝胶电泳中，血清白蛋白的测量范围分别为每管1至50微克和每板10至100微克。
