EcoRI甲基化酶导致DNA过度甲基化。
Overmethylation of DNAs by the EcoRI methylase.
作者信息
Berkner K L, Folk W R
出版信息
Nucleic Acids Res. 1978 Feb;5(2):435-50. doi: 10.1093/nar/5.2.435.
Abstract
EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI endonuclease activity, and thus is presumably at or near the EcoRI sequences (5') NAATTN.
摘要
EcoRI甲基化酶能够催化在非典型EcoRI位点的序列处将甲基掺入DNA。在高酶浓度以及广泛的pH和离子强度范围内，EcoRI甲基化酶会在多个位点修饰多瘤病毒DNA（其含有一个EcoRI位点）。这种修饰会阻止EcoRI核酸内切酶的活性，因此推测是在EcoRI序列（5'）NAATTN处或其附近。
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