弗氏柠檬酸杆菌RFL13中限制性内切酶Cfr13 I的特性分析。

Characterization of restriction-modification enzymes Cfr13 I from Citrobacter freundii RFL13.

作者信息

Bitinaité J B, Klimasauskas S J, Butkus V V, Janulaitis A A

出版信息

FEBS Lett. 1985 Mar 25;182(2):509-13. doi: 10.1016/0014-5793(85)80364-1.

Abstract

This communicatiopn describes some properties of RCfr13 I and MCfr13 I, isolated from Citrobacter freundii RFL13. RCFfr13 I restriction enzyme recognizes the 5'-G GNCC sequence and cleaves, as indicated by the arrow. MCfr13 I methylase modifies the internal cytosine producing m5C (5'-GGNm5CC). RCfr13 I is sensitive not only to this type of substrate modification but also to hemimethylation in overlapping sites by MCfr10 I (internal cytosine of RCfr13 I recognition is methylated) and MHpa II (external cytosine is methylated). From these results the sensitivity of RCfr13 I to methylation by dcm methylase of E.coli in overlapping sites is deduced.

摘要

本通讯描述了从弗氏柠檬酸杆菌RFL13中分离出的RCfr13 I和MCfr13 I的一些特性。RCFfr13 I限制酶识别5'-G GNCC序列并如箭头所示进行切割。MCfr13 I甲基化酶修饰内部胞嘧啶产生m5C（5'-GGNm5CC）。RCfr13 I不仅对这种类型的底物修饰敏感，而且对MCfr10 I（RCfr13 I识别序列的内部胞嘧啶被甲基化）和MHpa II（外部胞嘧啶被甲基化）在重叠位点的半甲基化也敏感。从这些结果可以推断出RCfr13 I对大肠杆菌dcm甲基化酶在重叠位点甲基化的敏感性。