Kifuji K
Arerugi. 1989 Nov;38(11):1226-35.
The percentage of histamine release from human leukocytes by challenge of Mycoplasma antigens and the level of Mycoplasma pneumoniae-specific IgE antibody in serum were examined to elucidate the mechanisms of Mycoplasma induced histamine release from human leukocytes. The following results were obtained. 1) In either normal persons (non-asthmatic group) or patients with bronchial asthma with and without increased titers of antibodies to M. pneumoniae IHA-positivity (IHA greater than or equal to 1:80) or negativity (IHA less than 1:80), histamine release from human leukocytes was found after challenge of Mycoplasma antigen. 2) In the non-asthmatic group, there was no significant difference in the histamine release rate among IHA-positive persons compared with that among IHA-negative persons. 3) In the asthmatic group too, IHA-positive and negative patients showed no significant difference in the histamine release rate. 4) The histamine release rate after challenge of M. pneumoniae increased in proportion to the concentration of the antigen added. 5) In all cases, the M. pneumoniae-specific IgE antibody (RAST) in serum was negative. These results suggest that histamine release from human leukocytes after challenge of Mycoplasma antigens occurs through non-immunological mechanisms that are not mediated by IgE antibody.
通过支原体抗原激发检测人白细胞组胺释放百分比及血清中肺炎支原体特异性IgE抗体水平，以阐明支原体诱导人白细胞组胺释放的机制。获得以下结果。1）在正常人（非哮喘组）或支气管哮喘患者中，无论肺炎支原体间接血凝试验（IHA）滴度升高（IHA大于或等于1:80）还是阴性（IHA小于1:80），支原体抗原激发后均发现人白细胞组胺释放。2）在非哮喘组中，IHA阳性者与IHA阴性者的组胺释放率无显著差异。3）在哮喘组中，IHA阳性和阴性患者的组胺释放率也无显著差异。4）肺炎支原体激发后的组胺释放率与添加抗原的浓度成正比。5）所有病例血清中肺炎支原体特异性IgE抗体（放射变应原吸附试验）均为阴性。这些结果表明，支原体抗原激发后人白细胞组胺释放是通过非免疫机制发生的，不受IgE抗体介导。
