Epand Richard M, D'Souza Kenneth, Berno Bob, Schlame Michael
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada.
Biochim Biophys Acta. 2015 Jan;1848(1 Pt B):220-8. doi: 10.1016/j.bbamem.2014.05.004. Epub 2014 May 13.
In addition to specific intermolecular interactions, biological processes at membranes are also modulated by the physical properties of the membrane. One of these properties is membrane curvature. NMR methods are useful for studying how membrane curvature affects the binding and insertion of proteins into membranes as well as how proteins can affect membrane curvature properties. In many cases these interactions result in a marked change in protein activity. We have reviewed examples from a range of systems having varied mechanisms by which membrane curvature is linked to protein activity. Among the examples discussed are antimicrobial peptides, proteins affecting membrane fusion, rhodopsin, protein kinase C, phospholipase C-delta1, phosphatidylinositol-3 kinase-related kinases and tafazzin.
除了特定的分子间相互作用外，膜上的生物过程还受到膜物理性质的调节。其中一个性质就是膜曲率。核磁共振方法对于研究膜曲率如何影响蛋白质与膜的结合和插入，以及蛋白质如何影响膜曲率性质很有用。在许多情况下，这些相互作用会导致蛋白质活性发生显著变化。我们回顾了一系列具有不同机制的系统中的例子，这些机制将膜曲率与蛋白质活性联系起来。讨论的例子包括抗菌肽、影响膜融合的蛋白质、视紫红质、蛋白激酶C、磷脂酶C-δ1、磷脂酰肌醇-3激酶相关激酶和tafazzin。
